##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1781309_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 147767 Sequences flagged as poor quality 0 Sequence length 76 %GC 90 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.834841338052474 32.0 27.0 32.0 13.0 32.0 2 27.519534131436654 32.0 27.0 32.0 13.0 32.0 3 27.27968355586836 32.0 27.0 32.0 13.0 32.0 4 26.775768608688 32.0 27.0 32.0 13.0 32.0 5 27.46272171729818 32.0 27.0 32.0 13.0 32.0 6 31.199442365345444 37.0 32.0 37.0 13.0 37.0 7 30.514675130441844 37.0 27.0 37.0 13.0 37.0 8 31.44534300621925 37.0 32.0 37.0 13.0 37.0 9 31.28865037525293 37.0 32.0 37.0 13.0 37.0 10-11 31.040076607090896 37.0 32.0 37.0 13.0 37.0 12-13 30.72591647661521 37.0 27.0 37.0 13.0 37.0 14-15 31.86776140816285 37.0 32.0 37.0 13.0 37.0 16-17 32.14510682358036 37.0 32.0 37.0 13.0 37.0 18-19 31.330929774577545 37.0 29.5 37.0 13.0 37.0 20-21 31.955318169821407 37.0 32.0 37.0 13.0 37.0 22-23 32.063549371645905 37.0 32.0 37.0 13.0 37.0 24-25 31.62770781026887 37.0 32.0 37.0 13.0 37.0 26-27 32.19296256945056 37.0 32.0 37.0 13.0 37.0 28-29 31.64806418212456 37.0 32.0 37.0 13.0 37.0 30-31 31.941096455906933 37.0 32.0 37.0 13.0 37.0 32-33 31.83704751399162 37.0 32.0 37.0 13.0 37.0 34-35 31.936454012059528 37.0 32.0 37.0 13.0 37.0 36-37 32.744083591058896 37.0 34.5 37.0 13.0 37.0 38-39 32.731797356649324 37.0 32.0 37.0 13.0 37.0 40-41 32.03035860510127 37.0 32.0 37.0 13.0 37.0 42-43 32.15813408947871 37.0 32.0 37.0 13.0 37.0 44-45 31.835132336719294 37.0 32.0 37.0 13.0 37.0 46-47 32.21387725270189 37.0 32.0 37.0 13.0 37.0 48-49 32.782448043203146 37.0 32.0 37.0 13.0 37.0 50-51 33.086287872123 37.0 34.5 37.0 13.0 37.0 52-53 32.25336171134286 37.0 32.0 37.0 13.0 37.0 54-55 32.391335683880705 37.0 32.0 37.0 13.0 37.0 56-57 32.14313073961033 37.0 32.0 37.0 13.0 37.0 58-59 31.685900776222027 37.0 32.0 37.0 13.0 37.0 60-61 31.391931216036056 37.0 32.0 37.0 13.0 37.0 62-63 30.94568814417292 37.0 29.5 37.0 13.0 37.0 64-65 30.563640731692463 37.0 27.0 37.0 13.0 37.0 66-67 30.560263793675176 37.0 27.0 37.0 13.0 37.0 68-69 30.934234301298666 37.0 27.0 37.0 13.0 37.0 70-71 31.102993225821734 37.0 27.0 37.0 13.0 37.0 72-73 31.71362347479478 37.0 32.0 37.0 13.0 37.0 74-75 32.22226207475282 37.0 32.0 37.0 13.0 37.0 76 32.78663707052319 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.262348314769941 11101 2 -0.5626875814986363 11101 3 -0.009946231040270703 11101 4 0.09329207981908993 11101 5 0.2352374473423069 11101 6 0.07829271389298498 11101 7 0.11760162137781194 11101 8 1.596124122520095 11101 9 0.026164562155603477 11101 10-11 0.5022738094163088 11101 12-13 -0.07493341935695241 11101 14-15 -0.24808712707787706 11101 16-17 0.008385658810027508 11101 18-19 0.45688296428955866 11101 20-21 -0.8542538275730216 11101 22-23 -0.5332642511608796 11101 24-25 0.19520245259904456 11101 26-27 -0.09190018142740541 11101 28-29 -1.1880415038890888 11101 30-31 0.3325979653566904 11101 32-33 -0.43074604707452835 11101 34-35 1.048819117205717 11101 36-37 0.3142374881123331 11101 38-39 -0.0127041916706645 11101 40-41 -0.17418982743696176 11101 42-43 -0.7473624166453483 11101 44-45 -0.19330871142382478 11101 46-47 -0.7110005183472161 11101 48-49 -0.008876425062595672 11101 50-51 -0.16045319032333083 11101 52-53 -0.03459416684717098 11101 54-55 -1.0026931986633087 11101 56-57 0.3300462397339743 11101 58-59 0.33313217167707165 11101 60-61 -0.3778443707579484 11101 62-63 0.35338779871672443 11101 64-65 1.311751165066152 11101 66-67 0.013185926613175525 11101 68-69 0.6880586651108942 11101 70-71 -0.5504139895756559 11101 72-73 -0.7287635633719738 11101 74-75 -0.9689628693291574 11101 76 -0.12056695326229772 11102 1 0.2114825139041372 11102 2 1.0325505137394586 11102 3 1.3347507386566981 11102 4 0.5045474910745007 11102 5 1.1275534646583232 11102 6 -0.5055817449814732 11102 7 -0.16865378987759883 11102 8 0.5073795337755094 11102 9 -1.2443982084071656 11102 10-11 0.7668841990266984 11102 12-13 -0.3909507353742683 11102 14-15 0.7497483707576222 11102 16-17 0.619316394740764 11102 18-19 0.657640540047133 11102 20-21 0.5415795057603141 11102 22-23 0.6707400778434547 11102 24-25 1.1243150067115941 11102 26-27 0.9557188661916456 11102 28-29 -1.5965912874388728 11102 30-31 -0.5911033333446056 11102 32-33 -0.4875642288927118 11102 34-35 0.22495548084207684 11102 36-37 -0.8286196547448057 11102 38-39 -0.14149207045854695 11102 40-41 0.6650958868487535 11102 42-43 0.36546225867932947 11102 44-45 0.04640990329479422 11102 46-47 -0.008619565966263565 11102 48-49 -0.07408205276821889 11102 50-51 0.32223079236064933 11102 52-53 -0.42636905862206476 11102 54-55 0.038161779691670006 11102 56-57 -0.332020859833122 11102 58-59 0.010621349166250127 11102 60-61 1.6659867980732201 11102 62-63 0.7400219978509242 11102 64-65 1.287129952944941 11102 66-67 -8.833374560879292E-4 11102 68-69 -0.1222768327246051 11102 70-71 -0.06258931425097813 11102 72-73 0.6751866530782387 11102 74-75 0.5616107237444368 11102 76 -1.1129911956865435 11103 1 0.6720363279062269 11103 2 -0.053656382812263814 11103 3 1.053272159764326 11103 4 1.344934115943886 11103 5 1.1449254604785928 11103 6 0.247422434746845 11103 7 1.0527411944484193 11103 8 -0.9309694735390153 11103 9 -0.2796646660874167 11103 10-11 -0.7308953203045441 11103 12-13 -0.6063373602436535 11103 14-15 0.18705244599273385 11103 16-17 1.2764480561827725 11103 18-19 0.3706760677378327 11103 20-21 0.08683812644996891 11103 22-23 1.1214976536010326 11103 24-25 1.3626901373281122 11103 26-27 -0.15203975449800566 11103 28-29 1.0695529131880868 11103 30-31 -0.07072715779601779 11103 32-33 0.8192539529254717 11103 34-35 -0.22847973336899585 11103 36-37 0.07224241422071032 11103 38-39 -0.08310649052123864 11103 40-41 0.045843013285534084 11103 42-43 0.7850677968193551 11103 44-45 0.6135467893971978 11103 46-47 0.6364953765624719 11103 48-49 0.39280666196531655 11103 50-51 -0.5011756862182395 11103 52-53 0.8923352255994672 11103 54-55 0.7783002331921907 11103 56-57 0.23870798029226847 11103 58-59 -0.13279557873343606 11103 60-61 0.9815562860565024 11103 62-63 0.2688229382897944 11103 64-65 -0.30893849010626084 11103 66-67 0.3947540711123523 11103 68-69 0.7529190920402868 11103 70-71 0.5222461089465114 11103 72-73 0.48285384221094674 11103 74-75 -0.8107116378020649 11103 76 -0.18665890728529178 11104 1 -1.2015268904845762 11104 2 -0.07664488855939311 11104 3 -1.4984519781667096 11104 4 -1.965410711642324 11104 5 0.018488678869399422 11104 6 -1.4882097491612036 11104 7 -1.6369139779653743 11104 8 -0.5171763700907377 11104 9 0.2490709660964896 11104 10-11 -0.41480336628155356 11104 12-13 -0.8707051763356048 11104 14-15 -0.1405337609038213 11104 16-17 -0.32125309324251816 11104 18-19 -0.8936917483541151 11104 20-21 -0.6085641724006088 11104 22-23 -1.9589621165139164 11104 24-25 -1.0281144603730432 11104 26-27 -1.1003156165669736 11104 28-29 -0.4706769718693842 11104 30-31 -0.9902673876810724 11104 32-33 -1.5428150125917668 11104 34-35 -1.4066406529092248 11104 36-37 -0.686378275434457 11104 38-39 -0.5256352261534261 11104 40-41 0.3331993351246183 11104 42-43 -0.37585174341053573 11104 44-45 -2.1048440152005057 11104 46-47 -0.6163781866559184 11104 48-49 -0.3600669012530773 11104 50-51 -1.173589479321688 11104 52-53 -0.35479121118214607 11104 54-55 -0.35388367485378325 11104 56-57 -1.4222115599376188 11104 58-59 -0.6327955787334361 11104 60-61 -1.4264896909549947 11104 62-63 -1.3863494755033088 11104 64-65 -0.7054902142441897 11104 66-67 -0.46731489440488616 11104 68-69 -0.8275406780746586 11104 70-71 -0.9835010174902692 11104 72-73 0.05181935945232752 11104 74-75 -2.029102442399765 11104 76 -1.7039002865956334 11105 1 -0.6008853507893086 11105 2 -1.1302401839349585 11105 3 -0.6766128977069386 11105 4 0.0653297320892996 11105 5 -0.8838101717053135 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11105 66-67 -0.5201750922129591 11105 68-69 -0.45036879889796566 11105 70-71 0.5567288675672017 11105 72-73 -1.3999983363509365 11105 74-75 -0.5056461472915039 11105 76 -1.0294816819444712 11106 1 -1.1037581278070903 11106 2 0.10902110197475423 11106 3 -1.1766128977069386 11106 4 -0.5032065196206865 11106 5 -1.783810171705312 11106 6 -1.252239498992168 11106 7 -0.2888409556530007 11106 8 0.5025666995509113 11106 9 -1.2703340372841723 11106 10-11 -0.773356442684527 11106 12-13 -0.7405496658555499 11106 14-15 -1.6662944099910426 11106 16-17 -0.1805499154196646 11106 18-19 -0.9999797808084807 11106 20-21 -0.6312146118867439 11106 22-23 -0.551051366006817 11106 24-25 -0.9554978275130033 11106 26-27 -0.11633995733776459 11106 28-29 -0.5393720361019732 11106 30-31 -2.4344188413659964 11106 32-33 -1.2807460470745298 11106 34-35 -1.0850044122060467 11106 36-37 -1.3036196547448071 11106 38-39 0.2789624749959998 11106 40-41 -0.4187276425630131 11106 42-43 -0.9519842653848443 11106 44-45 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24-25 0.7640570214536133 11107 26-27 -0.18279464732187023 11107 28-29 1.2326470417994564 11107 30-31 1.1226558804146052 11107 32-33 0.08952422319574183 11107 34-35 1.491386684773282 11107 36-37 -0.8590250601502092 11107 38-39 0.7857192317527577 11107 40-41 -0.270714923962057 11107 42-43 1.0219426026596707 11107 44-45 0.62441973130462 11107 46-47 0.7312452988986067 11107 48-49 1.1106538194676432 11107 50-51 -0.5346488145189525 11107 52-53 0.6436555114025069 11107 54-55 -0.10388367485378325 11107 56-57 0.6429484276361634 11107 58-59 2.041948130493033 11107 60-61 0.15738728947371428 11107 62-63 0.46406990939883386 11107 64-65 1.185625038940028 11107 66-67 1.038121576548825 11107 68-69 0.5735155505677803 11107 70-71 -0.9230008621625281 11107 72-73 0.016249306640890637 11107 74-75 -0.9990465244128117 11107 76 -1.5282246109199562 11108 1 -0.46480741238578815 11108 2 -1.7512332700443238 11108 3 -1.744180465274507 11108 4 -1.0415213049942942 11108 5 -1.2486750365701766 11108 6 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0.47944812768275114 21611 34-35 -0.9087051546389446 21611 36-37 -0.0676973246477175 21611 38-39 0.07507897985036749 21611 40-41 0.25854248879050346 21611 42-43 -0.19847198668695754 21611 44-45 0.13753964733715662 21611 46-47 -0.5219690805293702 21611 48-49 -0.062332273421361606 21611 50-51 0.5896623898893409 21611 52-53 -0.9303408150210046 21611 54-55 0.2771842863112681 21611 56-57 -0.15047849443153538 21611 58-59 0.3422630084778113 21611 60-61 0.1531889163433 21611 62-63 -1.6345918591658446 21611 64-65 -0.6607407443189608 21611 66-67 -1.8922116693183995 21611 68-69 -0.5798562678525805 21611 70-71 -1.0500672489376512 21611 72-73 -1.470499578166244 21611 74-75 -0.7162467343314702 21611 76 1.6456142765111608 21612 1 0.648563853617059 21612 2 0.6378136716341949 21612 3 -0.4081918450753612 21612 4 0.07512165375392854 21612 5 0.2898740388210008 21612 6 -1.0906295918714264 21612 7 1.0962983632324494 21612 8 1.359532643823357 21612 9 -0.7130584954885073 21612 10-11 0.9947550124238269 21612 12-13 0.7476856282620936 21612 14-15 1.088504351618866 21612 16-17 1.25412500718096 21612 18-19 1.0709180520088566 21612 20-21 0.21986897944452366 21612 22-23 -0.00832690780248413 21612 24-25 0.38567864307523436 21612 26-27 0.14124518198111957 21612 28-29 0.9699158895946205 21612 30-31 0.5045904465596962 21612 32-33 1.1139907950307375 21612 34-35 1.6527664856267705 21612 36-37 1.2069066610446626 21612 38-39 1.6631730013117902 21612 40-41 1.1716748342171783 21612 42-43 1.6282014931290902 21612 44-45 0.05286923343833294 21612 46-47 -0.5230932501767924 21612 48-49 -0.14603181353377437 21612 50-51 1.0901733760927144 21612 52-53 0.8937266351578401 21612 54-55 0.6198005356725318 21612 56-57 0.6408858387314709 21612 58-59 -0.20684276566628768 21612 60-61 -0.2832954985774947 21612 62-63 0.22907702177436562 21612 64-65 -0.06544181738998489 21612 66-67 -0.32133788291063325 21612 68-69 0.7304145548352032 21612 70-71 -0.7853763955906956 21612 72-73 0.3607727774801539 21612 74-75 0.5589193361846299 21612 76 1.748731292351735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 27.0 13 147.0 14 311.0 15 642.0 16 1094.0 17 1718.0 18 2217.0 19 2368.0 20 2580.0 21 2734.0 22 2920.0 23 3265.0 24 3456.0 25 3710.0 26 3934.0 27 4268.0 28 4607.0 29 5317.0 30 5765.0 31 6739.0 32 8006.0 33 10349.0 34 13771.0 35 21723.0 36 36096.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 81.71310238415884 3.3133243552349305 2.6535017967475825 12.320071463858643 2 79.03456116724303 3.711924854669852 10.915156970094811 6.338357007992313 3 77.50647979589489 3.8154662407709434 12.170511683934844 6.507542279399324 4 80.2161511027496 6.532581699567562 5.035630418158317 8.215636779524521 5 79.90078975684692 5.465361007532128 10.189013785215915 4.44483545040503 6 84.60143333761935 8.399033613729722 2.577706795157241 4.421826253493675 7 81.58046113137574 4.923291397944061 2.5736463486434724 10.922601122036719 8 82.87709705143908 9.064946841987725 2.6697435828026554 5.388212523770531 9 79.00410781838976 3.366110159913919 2.5046187579094115 15.12516326378691 10-11 82.41082244344136 5.850426685254488 5.734703959612092 6.004046911692056 12-13 80.94770821631352 3.6621843848761904 6.533935181738819 8.856172217071471 14-15 84.91984001840736 4.524352527966325 3.121129886916565 7.434677566709752 16-17 84.6119228244466 3.602631169340922 2.6206798540946217 9.16476615211786 18-19 81.55305311740781 3.4212645583926045 5.105334749978006 9.920347574221578 20-21 84.81325329742094 3.2409130590727293 3.33091962346126 8.61491402004507 22-23 84.42649576698452 3.129250779944101 2.499204829224387 9.945048623847002 24-25 83.25674880047642 5.048150128242436 2.402092483436762 9.293008587844378 26-27 85.24655619884209 5.130426249699694 2.682290521129763 6.94072703032846 28-29 83.50409766727348 5.619996345598137 4.672897196261682 6.203008790866702 30-31 83.53584878846017 3.642571218780982 4.735511770259159 8.086068222499687 32-33 86.0763905337457 3.3021581273220675 3.716323671726434 6.905127667205805 34-35 85.4054017473455 3.398932102566879 3.382690316511806 7.812975833575832 36-37 85.50517201124748 2.9641359848138786 5.419022579542725 6.111669424395922 38-39 85.20136431002862 3.042966291526525 5.977315638809748 5.778353759635102 40-41 84.9051879322713 5.041078461892452 4.024944845228267 6.028788760607989 42-43 83.41206087962806 4.924306509572503 5.678872820047777 5.9847597907516565 44-45 82.4598861721494 3.4743887336144064 6.3674568746743185 7.698268219561879 46-47 84.23193270486644 3.2761035955253877 4.447542414747542 8.044421284860626 48-49 84.69956079503542 3.3285510296615617 5.941109990728647 6.0307781845743635 50-51 86.17451799116176 3.411451812650998 4.466491165145127 5.947539031042114 52-53 82.56511941096456 3.7613269539206997 5.782075835606055 7.891477799508687 54-55 84.20080261492755 3.565410409631379 4.009690932346194 8.224096043094873 56-57 84.0718834381154 3.8198650578275255 3.8279859508550627 8.280265553202 58-59 82.74851624516975 5.562134982776939 5.5130712540689055 6.176277517984395 60-61 82.09579946808151 4.256701428600432 5.802378068174897 7.845121035143164 62-63 82.18608412596902 6.0592218127924795 5.493464350850836 6.261229710387672 64-65 81.58079950191856 6.284217721142069 6.360351093275224 5.774631683664147 66-67 81.85487964159792 8.689693910006971 3.4916456312979216 5.963780817097187 68-69 81.80919961831803 9.102505972240081 3.359342749057638 5.728951660384253 70-71 82.10730406653718 8.801694559678412 3.3667869009995464 5.724214472784857 72-73 83.0708688060853 7.802539149736747 3.4649378070733454 5.661654237104612 74-75 84.5499487366893 6.36071098659033 3.5728666511015685 5.516473625618797 76 86.86716249230207 4.046911692055737 3.4155122591647666 5.670413556477428 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 1.0 10 1.5 11 1.0 12 1.0 13 1.0 14 3.0 15 5.5 16 4.0 17 2.0 18 3.0 19 6.5 20 7.5 21 6.0 22 6.0 23 12.5 24 25.5 25 32.0 26 31.0 27 39.0 28 49.0 29 50.0 30 51.5 31 68.0 32 96.0 33 109.0 34 112.5 35 124.0 36 141.0 37 150.0 38 152.0 39 191.0 40 247.0 41 290.5 42 315.0 43 410.5 44 838.0 45 1699.0 46 2228.0 47 1789.0 48 942.5 49 465.0 50 395.0 51 395.0 52 432.5 53 510.0 54 550.0 55 591.0 56 722.0 57 880.5 58 949.0 59 1061.0 60 1258.0 61 1411.0 62 1479.0 63 1490.5 64 1487.0 65 1474.0 66 1444.0 67 1412.0 68 1380.5 69 1298.5 70 1190.0 71 1132.0 72 1127.5 73 1120.0 74 1138.0 75 1159.0 76 1103.0 77 989.0 78 842.0 79 753.0 80 720.0 81 672.5 82 623.0 83 588.0 84 593.5 85 588.0 86 594.5 87 612.0 88 634.5 89 712.0 90 847.0 91 1015.5 92 1104.0 93 1262.0 94 1660.0 95 2284.0 96 2668.0 97 3433.0 98 6278.0 99 49434.0 100 90510.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0010151116284420744 28-29 0.0 30-31 3.383705428140248E-4 32-33 0.0 34-35 0.0 36-37 3.383705428140248E-4 38-39 0.0 40-41 6.767410856280496E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.383705428140248E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 6.767410856280496E-4 74-75 3.383705428140248E-4 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 147767.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.597278147353602 #Duplication Level Percentage of deduplicated Percentage of total 1 88.06422065034857 23.422685714672422 2 6.322833443590657 3.3634031955714065 3 2.6868861635540178 2.143915759269661 4 1.3103658846878021 1.394086636393782 5 0.592845147829627 0.7884033647566777 6 0.25443997760928194 0.40604465137682977 7 0.12467558902854817 0.232122192370421 8 0.08650959238715586 0.1840735752908295 9 0.04834359574576357 0.11572272564239648 >10 0.3740267670856445 2.474165409056149 >50 0.08650959238715586 1.5389092287181847 >100 0.038165996641392294 2.2461036631994964 >500 0.005088799552185639 1.1484296223108001 >1k 0.0025443997760928195 1.7750918676023741 >5k 0.0 0.0 >10k+ 0.0025443997760928195 58.76684239376857 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 86838 58.76684239376857 No Hit CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG 2623 1.7750918676023741 No Hit CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 907 0.6138041646646409 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 790 0.5346254576461592 No Hit GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 493 0.33363335521462845 No Hit GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 304 0.20572929003092708 No Hit TCTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGT 294 0.19896187917464656 No Hit GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 292 0.19760839700339047 No Hit GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 290 0.19625491483213436 No Hit GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 229 0.15497370860882337 No Hit TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 220 0.1488830388381709 No Hit GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 204 0.13805518146812212 No Hit GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 202 0.136701699296866 No Hit CTCTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGG 167 0.11301576129988428 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0013534821712560992 0.0 5 0.0 0.0 0.0 0.006090669770652446 0.0 6 0.0 0.0 0.0 0.006090669770652446 0.0 7 0.0 0.0 0.0 0.006090669770652446 0.0 8 0.0 0.0 0.0 0.006090669770652446 0.0 9 0.0 0.0 0.0 0.009474375198792694 0.0 10 0.0 0.0 0.0 0.012181339541304893 0.0 11 0.0 0.0 0.0 0.01556504496944514 0.0 12 0.0 0.0 0.0 0.021655714740097587 0.0 13 0.0 0.0 0.0 0.021655714740097587 0.0 14 0.0 0.0 0.0 0.024362679082609785 0.0 15 0.0 0.0 0.0 0.024362679082609785 0.0 16 0.0 0.0 0.0 0.024362679082609785 0.0 17 0.0 0.0 0.0 0.025716161253865884 0.0 18 0.0 0.0 0.0 0.025716161253865884 0.0 19 0.0 0.0 0.0 0.026392902339493932 0.0 20 0.0 0.0 0.0 0.028423125596378083 0.0 21 0.0 0.0 0.0 0.03113008993889028 0.0 22 0.0 0.0 0.0 0.042634688394567126 0.0 23 0.0 0.0 0.0 0.04534165273707932 0.0 24 0.0 0.0 0.0 0.05887647444964031 0.0 25 0.0 0.0 0.0 0.06226017987778056 0.0 26 0.0 0.0 0.0 0.06361366204903666 0.0 27 0.0 0.0 0.0 0.09542049307355499 0.0 28 0.0 0.0 0.0 0.12587384192681722 0.0 29 0.0 0.0 0.0 0.1732457179207807 0.0 30 0.0 0.0 0.0 0.21114321871595146 0.0 31 0.0 0.0 0.0 0.2686662109943357 0.0 32 0.0 0.0 0.0 0.32415898001583576 0.0 33 0.0 0.0 0.0 0.35934951646849433 0.0 34 0.0 0.0 0.0 0.42905384828818344 0.0 35 0.0 0.0 0.0 0.5292115289611348 0.0 36 0.0 0.0 0.0 0.7024572468819155 0.0 37 0.0 0.0 0.0 0.9129237245122389 0.0 38 0.0 0.0 0.0 1.133541318426983 0.0 39 0.0 0.0 0.0 1.3717541805680564 0.0 40 0.0 0.0 0.0 1.6221483822504348 0.0 41 0.0 0.0 0.0 1.8535938335352278 0.0 42 0.0 0.0 0.0 2.034283703397917 0.0 43 0.0 0.0 0.0 2.2454269221138685 0.0 44 0.0 0.0 0.0 2.452509694316052 0.0 45 0.0 0.0 0.0 2.6338763052643688 0.0 46 0.0 0.0 0.0 2.8612613100353936 0.0 47 0.0 0.0 0.0 3.060899930295668 0.0 48 0.0 0.0 0.0 3.2537711396996625 0.0 49 0.0 0.0 0.0 3.408068107222858 0.0 50 0.0 0.0 0.0 3.5704859677735894 0.0 51 0.0 0.0 0.0 3.701096997299803 0.0 52 0.0 0.0 0.0 3.834414991168529 0.0 53 0.0 0.0 0.0 4.021872271887498 0.0 54 0.0 0.0 0.0 4.141655444043663 0.0 55 0.0 0.0 0.0 4.235045713860334 0.0 56 0.0 0.0 0.0 4.352121921673986 0.0 57 0.0 0.0 0.0 4.467167906230755 0.0 58 0.0 0.0 0.0 4.592365007071944 0.0 59 0.0 0.0 0.0 4.737187599396347 0.0 60 0.0 0.0 0.0 4.838698762240554 0.0 61 0.0 0.0 0.0 4.946300594855415 0.0 62 0.0 0.0 0.0 5.051195463127762 0.0 63 0.0 0.0 0.0 5.154060108143225 0.0 64 0.0 0.0 0.0 5.241359708189244 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACAAA 20 7.905805E-5 70.00001 66 GTAGATA 15 0.0022210588 70.0 38 ATCCAGT 15 0.0022210588 70.0 6 GCCTACA 15 0.0022210588 70.0 28 AGTATCA 15 0.0022210588 70.0 56 TCAGCGT 15 0.0022210588 70.0 28 GCAGTAT 15 0.0022210588 70.0 54 TGAAGCT 15 0.0022210588 70.0 13 ATCAATA 15 0.0022210588 70.0 59 GACTCAT 15 0.0022210588 70.0 55 GTGCTTG 15 0.0022210588 70.0 30 TGTGTAG 40 1.0586518E-8 61.250004 35 GTTGTGG 30 8.344154E-6 58.333332 1 GGTGGTA 25 2.3841996E-4 56.000004 47 AGCCGAC 25 2.3841996E-4 56.000004 19 AGATCTC 475 0.0 56.000004 40 CGCGTGT 25 2.3841996E-4 56.000004 7 TAGATCT 475 0.0 56.000004 39 CGTATCA 455 0.0 55.384617 56 GCCGTAT 455 0.0 55.384617 54 >>END_MODULE