FastQCFastQC Report
Wed 25 May 2016
SRR1781309_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781309_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147767
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2846819.265465225659316No Hit
CTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT84745.734703959612092No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT59354.016458343202474No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46783.165794798568016No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG43532.9458539457388997No Hit
CTAAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT43432.939086534882619No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC21081.4265702085039285No Hit
TCATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG16591.1227134610569343No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT16551.1200064967144219No Hit
CAAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT13570.9183376531972632No Hit
CATAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT12140.8215636779524521No Hit
CAAAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT11980.8107358205824033No Hit
TCAAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG7070.47845594753903103No Hit
TCTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG6210.4202562141750188No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC5540.3749145614379395No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT5400.36544018623914676No Hit
CTCATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC4550.30791719396076256No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTAAATCTCGTATGCCGT4190.28355451487815275No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT3860.2612220590524271RNA PCR Primer, Index 15 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC3530.23888960322670152No Hit
CTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT3420.23144545128479296No Hit
CTCTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC3250.21994085282911613No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT2760.18678053963334168No Hit
CTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT2730.18475031637645753No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT2540.17189223574952459No Hit
CTAAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT2520.1705387535782685No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT2470.16715504815012824No Hit
CTTATACACATCTCCGCGCCCACGAGACATAAGTTAATCTCGTATGCCGT2250.15226674426631115No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC2180.1475295566669148No Hit
CTCAAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC2090.14143888689626236No Hit
CTAATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT2040.13805518146812212No Hit
CTAAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT1960.1326412527830977No Hit
TCATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1940.13128777061184163No Hit
CTAATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT1920.12993428844058552No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAGTTAATCTCGTATGCCGT1840.12452035975556111No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1840.12452035975556111No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT1820.12316687758430502No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC1780.12045991324179282No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT1650.11166227912862818No Hit
ATACCCATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT1620.10963205587174404RNA PCR Primer, Index 15 (95% over 21bp)
CTAAAACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT1490.10083442175857937No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT1490.10083442175857937No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTAAT207.8382014E-570.121447
CCGTCAA252.7997612E-670.121447
AGTAATC150.002205839470.121448
GCAGTAA207.8513825E-570.09762646
AGCCGTA150.00220880970.09762645
TCTACAG401.3278623E-1070.07387550
ACAGCTT252.8110662E-670.07387553
AATCTGC455.456968E-1270.0738751
ACAGAAT150.002211781270.0738753
TCAACAG150.002211781270.0738750
ATATGCT150.002211781270.0738752
TAATATG150.002211781270.0738750
AAAGCAG150.002211781270.0738743
ACAGCTA150.002211781270.0738753
CTATGCA301.01306796E-770.0501342
AGCTTCA252.8224094E-670.0264155
TGATTAA150.00221773570.0264155
TGCTTTA150.00221773570.0264155
TTCTTGA252.8224094E-670.0264155
CTGCTTA455.456968E-1270.0264154
Produced by FastQC (version 0.11.5)