FastQCFastQC Report
Wed 25 May 2016
SRR1781308_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781308_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178441
Sequences flagged as poor quality0
Sequence length76
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4838527.115405091879108No Hit
CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG12760.7150822961090781No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6270.35137664550187453No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3500.19614326303932392No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2430.13617946548158774No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2300.1288941442829843No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGCG207.910299E-570.0000152
GGGTGTA150.002221816270.034
TCTAATG150.002221816270.09
ACATAGC150.002221816270.03
TTGTGCG308.35123E-658.3333322
TCTTGCG252.3855473E-455.99999664
TGATAGA200.006942461252.50000445
ATCTCTA200.006942461252.50000440
GTATTAC200.006942461252.5000041
CAGTGCC200.006942461252.5000044
CAACTAT200.006942461252.50000423
CATTTAC200.006942461252.50000445
GTATCAT2300.051.7391357
CGTATCA2350.050.63829856
TATCATT2250.049.7777858
CATTAAA2400.049.58333261
GCCGTAT2450.048.5714354
TCATTAA2400.048.12560
ATCATTA2400.048.12559
CCGTATC2550.046.66666855
Produced by FastQC (version 0.11.5)