FastQCFastQC Report
Wed 25 May 2016
SRR1781308_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781308_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178441
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT158288.870158763961197No Hit
CTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT46352.5974972119636184No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT31511.7658497766768848No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG26701.4962928923285568No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22861.28109571230827No Hit
CTAAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT22571.2648438419421546No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC12140.6803369180849693No Hit
TCATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG10250.5744195560437344No Hit
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT10020.5615301416154359No Hit
CAAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT8040.45056909566747555No Hit
CATAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT7340.41134044305961076No Hit
CAAAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT6900.3866824328489529No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC3600.20174735626901888No Hit
TCAAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG3480.19502244439338492No Hit
TCTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG3350.18773712319478147No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT2470.13842110277346575Illumina PCR Primer Index 9 (95% over 21bp)
CTCATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC2300.1288941442829843No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1800.10087367813450944No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAGA150.002211700770.0805547
CGCATTC150.002219098270.0215958
TTCTTGA207.898216E-570.0215955
CGTAAGA207.898216E-570.0215941
CTTAATA150.002224040269.9823461
CCTTATA150.002224040269.9823461
CATAGAT207.92019E-569.98234632
CAAGCTA401.3460522E-1069.98234628
CGCACTA207.92019E-569.98234639
ACGTAAG150.002224040269.98234640
ATAACCG252.8354061E-669.9823410
CTAAAAC5400.066.094441
AGGGGGG27050.064.5493370
AACACAT15400.064.0747455
AAACACA16500.062.9841084
AAAACAC9000.062.595323
TAAAACA5900.062.2724232
TAAGCCG1300.061.9943343
CTGCAAG401.0577423E-861.26889454
TAAACAC7950.060.739393
Produced by FastQC (version 0.11.5)