FastQCFastQC Report
Wed 25 May 2016
SRR1781307_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781307_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124807
Sequences flagged as poor quality0
Sequence length76
%GC81

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4541636.38898459221037No Hit
CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG12070.9670931918882755No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5340.42786061679232734No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3530.2828366998645909No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2460.19710432908410588No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1890.15143381380852033No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1400.11217319541371878No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGAC207.901E-570.05
GAGACAG207.901E-570.07
TGTAGGT207.901E-570.037
ACTCAGC150.002220247970.03
AGAGACA207.901E-570.06
CATTAAA1900.058.94736561
GTATCAT2150.058.60464557
CGTATCA2100.058.33333256
CCGTATC2050.058.04878255
GCCGTAT2050.058.04878254
TATCATT1950.057.43589858
TCATTAA2000.055.99999660
CATAAAA252.382759E-455.99999661
ATCATTA2000.055.99999659
TATAAGA252.382759E-455.9999962
TAGATCT2200.055.68181639
TTAAAAA2050.054.63414863
ATTAAAA2000.054.24999662
ATCTCGG2200.054.09090842
TCTCGGT2200.054.09090843
Produced by FastQC (version 0.11.5)