FastQCFastQC Report
Wed 25 May 2016
SRR1781307_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781307_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124807
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT119239.55315006369835No Hit
CTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT30912.476623907312891No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23111.851658961436458No Hit
CTTAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT20931.6769892714350958No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG20621.652150921022058No Hit
CTAAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT14031.1241356654674817No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC8810.7058898939963304No Hit
TCATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG6880.551251131747418No Hit
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT5840.4679224722972269No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT4530.36296041087439No Hit
CAAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT4420.3541468026633121No Hit
CATAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT3850.30847628738772664No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCAGGATCTCGTATGCCGT3560.28524041119488486No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT3420.274023091653513No Hit
CAAAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT3140.2515884525707693No Hit
CTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT2710.21713525683655566No Hit
TCAAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG2220.17787463844175405No Hit
TCTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG2150.17226597867106813No Hit
CTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT2120.16986226734077417No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC2110.16906103023067615No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGACGGATCTCGTATGCCGT2020.16184989623979426No Hit
CTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTAAGCCGT1580.12659546339548264No Hit
CTTAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT1450.11617938096420875No Hit
CTAAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT1440.11537814385411074No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGCCGGATCTCGTATGCCGT1370.10976948408342481No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC1360.10896824697332681No Hit
CTCATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC1340.10736577275313085No Hit
CTAAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT1320.10576329853293484No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTAAGCCGT1310.10496206142283684No Hit
CTTAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT1300.10416082431273888No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTATT150.002214351770.0469247
TAATACG150.002214351770.0469243
GAAAGAC301.0121039E-770.0469242
GGTATGC150.002214351770.0469242
GTATACC301.0121039E-770.0469242
ATACGTC150.002214351770.0469245
AATACGT150.002214351770.0469244
TGAAGTA252.8149352E-670.0469145
GAATGCA207.874804E-570.0469142
GCAGTAT207.874804E-570.0469146
GCAGTAA207.874804E-570.0469146
ACCTAAT207.874804E-570.0469147
CGACTTA455.456968E-1270.0469148
TATACCG252.8149352E-670.0469143
ACGACTT207.874804E-570.0469147
TGATAGA150.002217877270.01884555
TGCTTAA301.0149233E-770.01884555
CGAAAGA301.0149233E-770.01884541
CATCTTT150.002217877270.01884554
ATGCTTT150.002217877270.01884554
Produced by FastQC (version 0.11.5)