FastQCFastQC Report
Wed 25 May 2016
SRR1781306_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781306_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263598
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7055926.767653775825313No Hit
CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG35631.3516794512856698No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8460.3209432545011722No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6080.23065425382590155No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3950.14984939187702487No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3300.12519063118840051No Hit
TCTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGT3200.12139697569784293No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCCG150.002222997470.035
GACGTAT252.3876458E-455.99999654
CGTATCA7000.055.056
GCCGTAT6900.054.27536454
CCGTATC7000.053.555
TCATTAA6950.052.87769760
TCTCGGT6950.052.87769743
TTTCCGC200.006946122752.50000436
CAATGTT200.006946122752.50000456
GTCTTTC200.006946122752.5000041
CTTAGTC200.006946122752.50000456
AGTGTGC200.006946122752.5000048
TGCGTCC200.006946122752.50000442
GACTAGG200.006946122752.5000041
CATTAAA7150.051.8881161
ATCATTA6950.051.87050259
GTATCAT7250.051.65517457
TAGATCT7250.051.17241339
CGCCGTA7400.050.6081153
TATCATT7200.050.55555358