FastQCFastQC Report
Wed 25 May 2016
SRR1781306_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781306_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263598
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT243919.253105107019021No Hit
CTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT66412.5193666112792967No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61432.3304425678495284No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT45991.7447021601074364No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG37411.419206519017595No Hit
CTAAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT30491.1566855590710097No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC19120.7253469297946115No Hit
TCATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG13700.51973080220639No Hit
CATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT12030.4563767555140782No Hit
CAAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT9710.3683639481331421No Hit
CATAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT8310.31525277126533585No Hit
CAAAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT7640.2898352794786No Hit
TCAAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG5230.19840818215616204No Hit
TCTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG5120.19423516111654868No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC4990.1893034089788238No Hit
CTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT4230.1604716272505861No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT4000.15174621962230364No Hit
CTCATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC3530.133916038816683No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC2750.10432552599033376Illumina PCR Primer Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT2660.10091123604883193Illumina PCR Primer Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA252.8088944E-670.1087347
CGACTTA600.070.1087348
GACGAAT150.002209351970.1087346
ATCGTAA207.930919E-569.97571639
TAACCGA252.8407667E-669.97571611
ACAAGTG150.00222605869.97571612
AAAACAC13400.063.7092363
AGGGGGG35200.063.41549370
CGTAAGA501.4551915E-1163.04991541
AACACAT23650.062.8745845
CGTCATA1250.061.6956848
AAACACA25150.061.628714
CTTAAAC9000.061.4347841
TCGAATG3200.061.22875240
TAAAACA9800.061.050242
CGAATGC2650.060.80285341
CTAAAAC9700.060.2482571
ACGTAAG352.9742478E-759.97918340
CTCGAAT3300.059.37333339
TAATCTC3050.058.50428435
Produced by FastQC (version 0.11.5)