FastQCFastQC Report
Wed 25 May 2016
SRR1781305_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781305_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362846
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5986116.497632604465807No Hit
CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG14930.41146932858568097No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7720.21276243916151755No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4120.11354679395666481No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAA150.002223673770.0000154
GCCGTAT3450.054.78260854
CGTATCA3550.053.23943356
CGCCGTA3700.052.97297353
CCGTATC3750.052.2666755
GTAGATC3750.049.46666738
CGGTGGT4100.047.8048846
TCTCGGT3800.046.97368243
TAGATCT3800.046.97368239
TGGTCGC4200.046.66666449
GTATCAT3700.046.35135357
TCGGTGG4150.045.5421745
AGATCTC4050.044.9382740
TCGCCGT4400.043.75000452
TATCATT3750.042.93333458
ATCTCGG4000.042.87500442
GTGGTCG4750.042.048
TGTAGAT4550.040.037
GATCTCG4550.040.041
CTCGGTG4500.039.66666844
Produced by FastQC (version 0.11.5)