FastQCFastQC Report
Wed 25 May 2016
SRR1781305_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781305_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362846
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT218036.008885312226123No Hit
CTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT53731.4807935046824272No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT37931.045347061838907No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG31610.8711684847014987No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27130.7477001262243486No Hit
CTAAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT22450.61871978745804No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC16750.4616283492170232No Hit
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT11550.31831686169890255No Hit
TCATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG10490.28910336616636256No Hit
CAAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT8100.22323520171091868No Hit
CATAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT6910.1904389189904257No Hit
CAAAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT5940.16370581458800704No Hit
TCTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG3840.10583002155184293No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC3700.10197163534943199No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTTA150.002217826270.04648647
CGAGTAT150.002221468670.0175155
TAACGTA207.922321E-569.9982138
AGGGGGG33350.062.6434370
TAAGACG352.962297E-760.02328543
AACGTAT352.967954E-760.00673339
TCGTATC601.8189894E-1258.3398840
AACACAT21350.057.8593985
CCGACTT1750.056.03718647
GATACGT252.3907784E-455.99085224
AAACACA23300.055.8706934
CGTATGA4900.055.7282241
AAGCCGT2650.055.49322544
TCTCGTA65250.055.14034738
TCGTATG64850.055.11026840
CTCGTAT64350.054.9945239
ATCTCGT65600.054.95286637
CGTATGC61750.054.93681741
TATGCCG59800.054.62821643
GTATGCC60600.054.36927442
Produced by FastQC (version 0.11.5)