FastQCFastQC Report
Wed 25 May 2016
SRR1781304_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781304_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147865
Sequences flagged as poor quality0
Sequence length76
%GC84

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6702145.32580394278565No Hit
CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG21301.4405031616677375No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7370.4984276197883204No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5210.3523484259290569No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3520.23805498258546645No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2500.16907314104081425No Hit
TCTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGT2140.144726608730937No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2050.1386399756534677No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1890.12781929462685557No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1570.10617793257363134No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTAGT150.002221061470.04
CTTGTAC207.905825E-570.01
TTGGGCG308.344181E-658.3333322
TTGTACG252.3842049E-456.0000042
AACTCCT252.3842049E-456.00000428
CGTGTGC252.3842049E-456.0000041
CGTTGCG252.3842049E-456.00000417
AACTGGC200.006940121852.528
TGTTCAG200.006940121852.565
ACCGTGT200.006940121852.570
GCTGACT200.006940121852.550
CGCGTCT608.367351E-1152.57
GTTGTGA200.006940121852.51
CGTATCA3450.050.7246456
TAGATCT3750.050.439
TATCATT3200.050.312558
CCGTATC3350.050.14925455
GCCGTAT3350.050.14925454
TGTACGC352.0785763E-550.0000043
CATTAAA3150.049.99999661
Produced by FastQC (version 0.11.5)