##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1781304_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 147865 Sequences flagged as poor quality 0 Sequence length 76 %GC 84 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.505028235214553 32.0 27.0 32.0 13.0 32.0 2 28.204321509485002 32.0 27.0 32.0 13.0 32.0 3 27.883109593210023 32.0 27.0 32.0 13.0 32.0 4 27.3158218645386 32.0 27.0 32.0 13.0 32.0 5 28.02532039360227 32.0 27.0 32.0 13.0 32.0 6 31.845913502181045 37.0 32.0 37.0 13.0 37.0 7 31.30518378250431 37.0 32.0 37.0 13.0 37.0 8 32.06512021100328 37.0 32.0 37.0 13.0 37.0 9 31.981685997362458 37.0 32.0 37.0 13.0 37.0 10-11 31.64014472660873 37.0 32.0 37.0 13.0 37.0 12-13 31.355560815608833 37.0 29.5 37.0 13.0 37.0 14-15 32.43771683630338 37.0 32.0 37.0 13.0 37.0 16-17 32.62480979271633 37.0 34.5 37.0 13.0 37.0 18-19 31.831782369052853 37.0 29.5 37.0 13.0 37.0 20-21 32.349572244953166 37.0 32.0 37.0 13.0 37.0 22-23 32.43089980725662 37.0 32.0 37.0 13.0 37.0 24-25 32.04993406147499 37.0 32.0 37.0 13.0 37.0 26-27 32.624045582118825 37.0 32.0 37.0 13.0 37.0 28-29 32.05543232002164 37.0 32.0 37.0 13.0 37.0 30-31 32.25765732255774 37.0 32.0 37.0 13.0 37.0 32-33 32.11788793832211 37.0 32.0 37.0 13.0 37.0 34-35 32.19801508132418 37.0 32.0 37.0 13.0 37.0 36-37 32.93965779596253 37.0 34.5 37.0 13.0 37.0 38-39 32.848486795387686 37.0 34.5 37.0 13.0 37.0 40-41 32.188100632333544 37.0 32.0 37.0 13.0 37.0 42-43 32.31988638284922 37.0 32.0 37.0 13.0 37.0 44-45 32.01740438913874 37.0 32.0 37.0 13.0 37.0 46-47 32.332982788354244 37.0 32.0 37.0 13.0 37.0 48-49 32.860014202143844 37.0 34.5 37.0 13.0 37.0 50-51 33.11982213505563 37.0 34.5 37.0 13.0 37.0 52-53 32.243938727893685 37.0 32.0 37.0 13.0 37.0 54-55 32.371409731849994 37.0 32.0 37.0 13.0 37.0 56-57 32.18839820106178 37.0 32.0 37.0 13.0 37.0 58-59 31.743397693842358 37.0 32.0 37.0 13.0 37.0 60-61 31.482730869374095 37.0 32.0 37.0 13.0 37.0 62-63 31.03227268116187 37.0 29.5 37.0 13.0 37.0 64-65 30.726071078348493 37.0 27.0 37.0 13.0 37.0 66-67 30.796723362526627 37.0 27.0 37.0 13.0 37.0 68-69 31.147468975078617 37.0 27.0 37.0 13.0 37.0 70-71 31.305819497514626 37.0 29.5 37.0 13.0 37.0 72-73 31.852831975112434 37.0 32.0 37.0 13.0 37.0 74-75 32.202485375173296 37.0 32.0 37.0 13.0 37.0 76 32.57526798092855 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.8673226518799879 11101 2 -1.5904923201192567 11101 3 -0.8628162973926798 11101 4 0.24785689343537598 11101 5 -1.3714970392993315 11101 6 -1.1204304453462832 11101 7 0.41703061251749673 11101 8 -1.3822391746505396 11101 9 -0.43321757078512846 11101 10-11 0.08555016554610617 11101 12-13 1.6547036144223597 11101 14-15 -0.41235929317262077 11101 16-17 -0.311851931406224 11101 18-19 -0.215896618161608 11101 20-21 1.3270048948373514 11101 22-23 0.8320873718210109 11101 24-25 0.20010971745022488 11101 26-27 0.4363796531365125 11101 28-29 0.6612606302207062 11101 30-31 0.3380145771958283 11101 32-33 0.8803017420313708 11101 34-35 -0.01791666783364576 11101 36-37 0.918420527014483 11101 38-39 0.22968598339885915 11101 40-41 -0.9033376904635944 11101 42-43 -0.4940230543902402 11101 44-45 1.4417820494180305 11101 46-47 0.544380310956555 11101 48-49 -1.074532492821973 11101 50-51 0.5536038040358306 11101 52-53 -0.4849761413809901 11101 54-55 -0.4252120586348127 11101 56-57 -0.6348454290890118 11101 58-59 0.08954729188769761 11101 60-61 1.2390144774737841 11101 62-63 -0.672029478055137 11101 64-65 -1.2375895393687522 11101 66-67 -0.17645752390450298 11101 68-69 -1.4952251808967176 11101 70-71 -0.9063279733967136 11101 72-73 -0.5087091449141248 11101 74-75 -1.1007195277656194 11101 76 -0.7598564596768576 11102 1 -0.16094319991527328 11102 2 0.31821661800144696 11102 3 0.06709202957447147 11102 4 1.02936949847739 11102 5 -1.047967627534625 11102 6 0.12173914976448685 11102 7 0.46439501282307205 11102 8 -0.2552338270569585 11102 9 -0.550482658638451 11102 10-11 -0.5692435701299843 11102 12-13 0.2676715288608591 11102 14-15 -0.4244868714766703 11102 16-17 -0.18217278702120154 11102 18-19 0.0036411969644412068 11102 20-21 -0.3754397193719683 11102 22-23 -0.23389811328211607 11102 24-25 0.13059100087268405 11102 26-27 -0.6354308892622704 11102 28-29 0.5501070778906829 11102 30-31 0.9835455168444156 11102 32-33 -0.9028342396340214 11102 34-35 -0.17624363498414297 11102 36-37 -0.564868243038994 11102 38-39 -0.5032208156844149 11102 40-41 -0.4892811587600008 11102 42-43 1.3811679310948648 11102 44-45 0.919627733146072 11102 46-47 0.6663245431949036 11102 48-49 -1.2158617517982933 11102 50-51 -1.4380883273621805 11102 52-53 0.17535617336308462 11102 54-55 -0.31148020225589335 11102 56-57 -1.0586422205328603 11102 58-59 -0.47433735287929935 11102 60-61 0.9490985110872323 11102 62-63 -0.650161640010829 11102 64-65 0.34243337888946 11102 66-67 0.3747265861031366 11102 68-69 0.19060369610863148 11102 70-71 0.3261013619738016 11102 72-73 -0.30024848792405834 11102 74-75 -0.3962695659627187 11102 76 0.4928173371145874 11103 1 -0.5666996056716798 11103 2 -0.08174828196345274 11103 3 0.5660390285214696 11103 4 -1.1019046327967388 11103 5 -0.8858054653724636 11103 6 -0.2722587442334081 11103 7 0.22466177308983148 11103 8 -0.8707129425360733 11103 9 0.5890399808841877 11103 10-11 0.5479905471041349 11103 12-13 -1.654318643129315 11103 14-15 0.5139125669227695 11103 16-17 -0.2061285609769712 11103 18-19 -0.9239649806417347 11103 20-21 -0.20757380150604376 11103 22-23 0.05883617945217878 11103 24-25 -0.2692861490044649 11103 26-27 0.37079936696798654 11103 28-29 -0.503683725900121 11103 30-31 -0.5948153115164132 11103 32-33 0.328036241236461 11103 34-35 -1.0624317711722782 11103 36-37 0.32640939823865267 11103 38-39 0.6694713570077653 11103 40-41 -0.23322640270524886 11103 42-43 -1.3869085869816544 11103 44-45 0.185422748941086 11103 46-47 -0.20661332974296442 11103 48-49 0.9783291923926498 11103 50-51 1.3026101633363112 11103 52-53 0.8852543832612909 11103 54-55 0.25503436425866965 11103 56-57 1.8166649047742638 11103 58-59 -1.4344988130407614 11103 60-61 -0.02290921092049203 11103 62-63 0.19899595914677093 11103 64-65 -1.0356817492256667 11103 66-67 -0.0711316097550565 11103 68-69 -0.1748754193704869 11103 70-71 -0.30815627228383136 11103 72-73 -0.672659860335429 11103 74-75 -0.32826324795640005 11103 76 -1.571064726767478 11104 1 0.3455503065782324 11104 2 -0.5129522131673845 11104 3 -1.101739139256697 11104 4 -0.9186824495745576 11104 5 -0.6843312638982617 11104 6 -1.9951439671186293 11104 7 -0.6395010910730328 11104 8 -0.32016889199202225 11104 9 -0.680352788508582 11104 10-11 -1.556256557142973 11104 12-13 -1.180380419191092 11104 14-15 -1.138772585762382 11104 16-17 -1.2341208389692504 11104 18-19 -2.4444107510875064 11104 20-21 -0.829985173917418 11104 22-23 -1.5910409704249737 11104 24-25 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-1.9909832045271223 11105 66-67 0.03281849419504468 11105 68-69 -1.2045011989962653 11105 70-71 -1.7788769930045554 11105 72-73 0.026924339248768803 11105 74-75 -0.8237587434518971 11105 76 -1.1365532922560462 11106 1 1.0080523614853512 11106 2 -0.40248040649304784 11106 3 0.10572428339659723 11106 4 -0.10806268958770815 11106 5 0.5976019927185412 11106 6 0.8572315055100077 11106 7 -0.7951808543477306 11106 8 -0.23797260726409064 11106 9 -0.840142366019677 11106 10-11 -0.9314825969259743 11106 12-13 -1.0131109797697562 11106 14-15 -0.7974114152872858 11106 16-17 0.010002126672702616 11106 18-19 -1.294731316597943 11106 20-21 -0.08364290858781231 11106 22-23 -1.1761963214031113 11106 24-25 0.6334678708381674 11106 26-27 -0.8186464079208235 11106 28-29 0.08158825494148303 11106 30-31 -0.35148653970662735 11106 32-33 -0.7811837382366846 11106 34-35 0.9889708973189997 11106 36-37 -0.3703918333727074 11106 38-39 -1.0161256323866965 11106 40-41 -1.1386318081106523 11106 42-43 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11107 22-23 0.26051253935639096 11107 24-25 0.23473369362297802 11107 26-27 0.5167966300538609 11107 28-29 0.9107021789921141 11107 30-31 -0.08566375489650113 11107 32-33 0.5352719579658469 11107 34-35 0.09023672010381034 11107 36-37 -0.5919108207144816 11107 38-39 -0.1237205690955534 11107 40-41 0.42465933112985255 11107 42-43 -1.1687252137349908 11107 44-45 -0.20415987165419125 11107 46-47 0.13550093642193417 11107 48-49 0.46455909317951694 11107 50-51 -0.99135151986588 11107 52-53 -1.9536097973750373 11107 54-55 0.22715205156622886 11107 56-57 -1.5989184000495484 11107 58-59 -0.44014369843248247 11107 60-61 -0.5472848523847453 11107 62-63 -0.5686229255607209 11107 64-65 -0.08178164659138787 11107 66-67 0.4590599742339947 11107 68-69 -2.595932552452936 11107 70-71 0.6251314458140129 11107 72-73 -0.014945183633411574 11107 74-75 -1.601184903789445 11107 76 -1.8006233993641274 11108 1 -0.8906818157298382 11108 2 -0.37716395079684517 11108 3 -0.9828833115401103 11108 4 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1.6110059942662645 11109 44-45 -1.642822162637156 11109 46-47 0.0506439275142867 11109 48-49 0.9817492791460722 11109 50-51 0.8530772971367782 11109 52-53 1.3373731687134196 11109 54-55 0.25770370026133804 11109 56-57 0.6220536268296541 11109 58-59 1.184953973078695 11109 60-61 0.122819851475235 11109 62-63 1.2866669634844428 11109 64-65 -0.3924551893324413 11109 66-67 0.39108060245715137 11109 68-69 1.7551379273095264 11109 70-71 1.5760612786003847 11109 72-73 0.7254048710626328 11109 74-75 0.08056224420242586 11109 76 -0.32648681552289815 11110 1 -0.1389518579239315 11110 2 -0.3226491828643532 11110 3 0.23973272221516595 11110 4 -0.932535263427372 11110 5 -0.7413009608679566 11110 6 -0.22631279828745932 11110 7 -0.1821850737570152 11110 8 0.3132510214278881 11110 9 -0.19204110019689224 11110 10-11 -0.20768512857154064 11110 12-13 -0.4165708953815681 11110 14-15 -0.8450063519961475 11110 16-17 -0.33793036277877775 11110 18-19 -0.6954064220831775 11110 20-21 -1.6094656933979365 11110 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-1.2509607295026797 21611 60-61 0.0010465630352811672 21611 62-63 -0.9021096919588807 21611 64-65 -0.4640601276040428 21611 66-67 -1.6850136736371226 21611 68-69 -0.31848721298228 21611 70-71 -2.307664871792433 21611 72-73 -0.05349524117081117 21611 74-75 0.20048368079052636 21611 76 -0.18900084470359957 21612 1 0.5051222161501414 21612 2 0.5080915578763872 21612 3 0.2134364259188679 21612 4 0.7489462180541118 21612 5 0.5075879280209321 21612 6 2.3218353498606845 21612 7 1.0259630743911359 21612 8 1.5376954658723392 21612 9 0.7116626035068094 21612 10-11 0.8352778343914196 21612 12-13 1.0378735490628799 21612 14-15 1.053326981337186 21612 16-17 1.0661437112952967 21612 18-19 0.9679269112501565 21612 20-21 0.9051639362316948 21612 22-23 0.5268041474201439 21612 24-25 0.9757088459905319 21612 26-27 0.7479264940951111 21612 28-29 0.9232432010268106 21612 30-31 1.0229274312263357 21612 32-33 1.1338420470422648 21612 34-35 0.4932489095084023 21612 36-37 0.8299129017421549 21612 38-39 0.9546315171679254 21612 40-41 2.078960784481943 21612 42-43 1.1372405621674986 21612 44-45 1.6179803064986444 21612 46-47 0.536755038625401 21612 48-49 0.6746505137139707 21612 50-51 1.0922748280009742 21612 52-53 -0.2660218930149796 21612 54-55 0.6635678977922055 21612 56-57 0.10970794781730575 21612 58-59 0.6448305162885681 21612 60-61 1.0271408391295545 21612 62-63 1.0937657289165372 21612 64-65 1.4146435760996567 21612 66-67 1.5670065283830823 21612 68-69 1.4094589149638495 21612 70-71 1.7951970810695173 21612 72-73 0.9738616611860884 21612 74-75 1.5975375528444005 21612 76 2.050056394353639 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 37.0 13 94.0 14 201.0 15 363.0 16 585.0 17 1054.0 18 1347.0 19 1692.0 20 1878.0 21 2243.0 22 2608.0 23 3024.0 24 3364.0 25 3797.0 26 4226.0 27 4635.0 28 4982.0 29 5685.0 30 6764.0 31 7997.0 32 9528.0 33 12493.0 34 16917.0 35 23357.0 36 28992.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 74.19740979947925 5.6111994048625435 3.617488925709262 16.57390186994894 2 66.87857167010449 7.648868900686437 13.076116728096574 12.396442701112502 3 65.6375748148649 7.0036857944746895 13.19987826733845 14.15886112332195 4 66.47482500929902 7.5623034524735395 7.73678693402766 18.226084604199777 5 66.81838163189396 9.892807628580123 12.779900584993067 10.50891015453285 6 74.80607310722618 10.821357319176276 4.725256145808677 9.647313427788863 7 71.60585669360565 8.005951374564637 4.602847191695127 15.785344740134583 8 71.30558279511718 12.136070063909647 5.378554762790383 11.179792378182801 9 66.76360193419674 5.617962330504176 4.78612247658337 22.83231325871572 10-11 71.63382691585878 8.268380848682408 7.619475939119938 12.478316296338878 12-13 69.40148108071553 6.507287052378858 8.465830318195652 15.625401548709972 14-15 72.28823589084638 6.633077469313226 5.5783992155006255 15.50028742433977 16-17 72.52223311804687 6.378115172623677 5.215568254827038 15.884083454502418 18-19 70.05173638115849 6.720995502654448 6.88330571805363 16.34396239813343 20-21 72.36398065803267 6.423088628140533 5.74341460115646 15.469516112670343 22-23 72.54759408920299 6.116728096574578 4.934230548135123 16.40144726608731 24-25 71.59706489027154 7.195076590132891 4.821289689919859 16.386568829675717 26-27 72.88574037128461 7.370912656815339 5.558786731139891 14.184560240760153 28-29 71.72048828323132 8.036046393669901 6.739255401886856 13.504209921211915 30-31 71.72792750143712 6.4213978967301255 6.625638251107429 15.225036350725324 32-33 73.5414060122409 6.426808237243431 6.123152875934129 13.908632874581544 34-35 72.85733608358976 6.465356913400737 5.967943732458662 14.70936327055084 36-37 72.73212975393012 5.811739802319015 7.703336500647552 13.752793943103313 38-39 72.13776079532006 5.976397389510702 8.773543434889932 13.112298380279308 40-41 71.9287863929936 7.286376086294931 7.4436141074628885 13.34122341324857 42-43 70.78652825212187 7.094308998072567 8.66499847834173 13.454164271463837 44-45 69.44679268251446 6.578974064180164 9.581713049065026 14.392520204240355 46-47 70.60663443005444 6.638487809826531 8.095560139316268 14.659317620802758 48-49 70.72194231224428 6.92286883305718 9.089710208636257 13.265478646062286 50-51 71.3694924424306 7.008419842423833 8.275792107665776 13.346295607479796 52-53 68.86078517566699 7.777364487877455 9.09884015825246 14.26301017820309 54-55 70.07980252257127 7.2275386332127285 7.573800426064316 15.118858418151692 56-57 70.34118959862037 7.457478105028235 7.2684543333446054 14.932877963006796 58-59 69.54282622662564 8.796199235789402 8.494910898454672 13.166063639130288 60-61 69.16173536671964 7.48960200182599 8.445879687552834 14.902782943901533 62-63 69.2578991505708 8.767177947302928 8.42902937834767 13.545893523778608 64-65 69.0115984174754 9.104588645047848 8.759341291042505 13.124471646434246 66-67 69.15463429479593 10.753389916477868 6.614141277516654 13.477834511209549 68-69 69.04406046055523 11.195685253440638 6.648294052006898 13.111960233997227 70-71 69.43022351469246 10.911980522774153 6.892435667669834 12.765360294863559 72-73 69.97700605281845 9.938119230379062 7.032428228451629 13.05244648835086 74-75 71.41717106820411 9.07178845568593 7.112568897304975 12.39847157880499 76 73.69492442430595 6.959726777804078 6.754133838298449 12.591214959591518 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.5 14 0.5 15 3.0 16 5.0 17 4.0 18 2.5 19 2.0 20 4.0 21 5.0 22 6.0 23 7.5 24 14.0 25 20.0 26 20.5 27 21.5 28 36.0 29 50.0 30 58.0 31 64.0 32 76.0 33 90.0 34 82.0 35 92.5 36 130.0 37 149.0 38 152.0 39 181.0 40 215.0 41 267.5 42 312.0 43 371.5 44 671.5 45 1375.5 46 1839.0 47 1611.5 48 986.0 49 572.0 50 556.0 51 590.0 52 665.5 53 824.5 54 942.0 55 1073.0 56 1320.0 57 1646.0 58 1856.0 59 1958.5 60 2277.5 61 2701.5 62 2909.0 63 3062.0 64 3239.5 65 3189.5 66 3081.0 67 3047.0 68 3021.0 69 2947.5 70 2849.0 71 2798.0 72 2816.5 73 2871.0 74 2774.0 75 2641.0 76 2495.5 77 2241.0 78 1979.0 79 1826.0 80 1642.5 81 1344.0 82 1069.0 83 909.0 84 789.0 85 624.5 86 545.5 87 511.0 88 494.0 89 486.5 90 557.0 91 672.5 92 727.0 93 841.5 94 1115.0 95 1536.5 96 1799.0 97 2306.5 98 4304.5 99 37890.5 100 69986.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.381462820816285E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.381462820816285E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 6.76292564163257E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 147865.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.23169783248233 #Duplication Level Percentage of deduplicated Percentage of total 1 82.43177660805615 29.866432218577753 2 8.591854257662298 6.225949345686945 3 4.7205734124762015 5.131031684306631 4 2.2492253705155485 3.2597301592668986 5 0.931421958412663 1.6873499475873261 6 0.43117930339343713 0.9373414939302741 7 0.20905663194833315 0.5302133703039935 8 0.057863889199985065 0.16772055591248775 9 0.044797849703214244 0.14607919385926352 >10 0.27252025236122 2.302099888411727 >50 0.031731810206443424 0.7831467893010516 >100 0.02053234778063986 1.3458222026848816 >500 0.003733154141934521 0.8507760457173774 >1k 0.0018665770709672604 1.4405031616677375 >5k 0.0 0.0 >10k+ 0.0018665770709672604 45.32580394278565 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 67021 45.32580394278565 No Hit CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG 2130 1.4405031616677375 No Hit CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 737 0.4984276197883204 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 521 0.3523484259290569 No Hit GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 352 0.23805498258546645 No Hit GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 250 0.16907314104081425 No Hit TCTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGT 214 0.144726608730937 No Hit GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 205 0.1386399756534677 No Hit GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 189 0.12781929462685557 No Hit GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 157 0.10617793257363134 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.016907314104081427 0.0 2 0.0 0.0 0.0 0.016907314104081427 0.0 3 0.0 0.0 0.0 0.016907314104081427 0.0 4 0.0 0.0 0.0 0.019612484360734452 0.0 5 0.0 0.0 0.0 0.026375410002367025 0.0 6 0.0 0.0 0.0 0.026375410002367025 0.0 7 0.0 0.0 0.0 0.026375410002367025 0.0 8 0.0 0.0 0.0 0.026375410002367025 0.0 9 0.0 0.0 0.0 0.027727995130693538 0.0 10 0.0 0.0 0.0 0.027727995130693538 0.0 11 0.0 0.0 0.0 0.02975687282318331 0.0 12 0.0 0.0 0.0 0.02975687282318331 0.0 13 0.0 0.0 0.0 0.02975687282318331 0.0 14 0.0 0.0 0.0 0.030433165387346566 0.0 15 0.0 0.0 0.0 0.031109457951509822 0.0 16 0.0 0.0 0.0 0.031109457951509822 0.0 17 0.0 0.0 0.0 0.031109457951509822 0.0 18 0.0 0.0 0.0 0.032462043079836335 0.0 19 0.0 0.0 0.0 0.03651979846481588 0.0 20 0.0 0.0 0.0 0.04125384641395868 0.0 21 0.0 0.0 0.0 0.05004564974808102 0.0 22 0.0 0.0 0.0 0.06019003821052987 0.0 23 0.0 0.0 0.0 0.06154262333885639 0.0 24 0.0 0.0 0.0 0.06627667128799919 0.0 25 0.0 0.0 0.0 0.0723633043654685 0.0 26 0.0 0.0 0.0 0.07980252257126433 0.0 27 0.0 0.0 0.0 0.12511412437020256 0.0 28 0.0 0.0 0.0 0.17515977411828357 0.0 29 0.0 0.0 0.0 0.23196834950799716 0.0 30 0.0 0.0 0.0 0.31177087207926146 0.0 31 0.0 0.0 0.0 0.3936022723430156 0.0 32 0.0 0.0 0.0 0.5072194231224427 0.0 33 0.0 0.0 0.0 0.6505934467250533 0.0 34 0.0 0.0 0.0 0.9299022757244784 0.0 35 0.0 0.0 0.0 1.24167314780374 0.0 36 0.0 0.0 0.0 1.652859026815 0.0 37 0.0 0.0 0.0 2.1553444019883004 0.0 38 0.0 0.0 0.0 2.6869103574206203 0.0 39 0.0 0.0 0.0 3.1921009028505734 0.0 40 0.0 0.0 0.0 3.672944915970649 0.0 41 0.0 0.0 0.0 4.110506204984276 0.0 42 0.0 0.0 0.0 4.558211882460352 0.0 43 0.0 0.0 0.0 5.004564974808102 0.0 44 0.0 0.0 0.0 5.451594359720015 0.0 45 0.0 0.0 0.0 5.928380617455111 0.0 46 0.0 0.0 0.0 6.345653129543841 0.0 47 0.0 0.0 0.0 6.720995502654448 0.0 48 0.0 0.0 0.0 7.067933588070199 0.0 49 0.0 0.0 0.0 7.386467385791093 0.0 50 0.0 0.0 0.0 7.652250363507253 0.0 51 0.0 0.0 0.0 7.911270415581781 0.0 52 0.0 0.0 0.0 8.131741791499003 0.0 53 0.0 0.0 0.0 8.385351503060225 0.0 54 0.0 0.0 0.0 8.62543536333818 0.0 55 0.0 0.0 0.0 8.87431102695026 0.0 56 0.0 0.0 0.0 9.094106110303317 0.0 57 0.0 0.0 0.0 9.282791735704865 0.0 58 0.0 0.0 0.0 9.466743313157272 0.0 59 0.0 0.0 0.0 9.704122003178576 0.0 60 0.0 0.0 0.0 9.938795522943225 0.0 61 0.0 0.0 0.0 10.220809522199303 0.0 62 0.0 0.0 0.0 10.393940418625098 0.0 63 0.0 0.0 0.0 10.593446725053258 0.0 64 0.0 0.0 0.0 10.807155175328846 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTAGT 15 0.0022210614 70.0 4 CTTGTAC 20 7.905825E-5 70.0 1 TTGGGCG 30 8.344181E-6 58.333332 2 TTGTACG 25 2.3842049E-4 56.000004 2 AACTCCT 25 2.3842049E-4 56.000004 28 CGTGTGC 25 2.3842049E-4 56.000004 1 CGTTGCG 25 2.3842049E-4 56.000004 17 AACTGGC 20 0.0069401218 52.5 28 TGTTCAG 20 0.0069401218 52.5 65 ACCGTGT 20 0.0069401218 52.5 70 GCTGACT 20 0.0069401218 52.5 50 CGCGTCT 60 8.367351E-11 52.5 7 GTTGTGA 20 0.0069401218 52.5 1 CGTATCA 345 0.0 50.72464 56 TAGATCT 375 0.0 50.4 39 TATCATT 320 0.0 50.3125 58 CCGTATC 335 0.0 50.149254 55 GCCGTAT 335 0.0 50.149254 54 TGTACGC 35 2.0785763E-5 50.000004 3 CATTAAA 315 0.0 49.999996 61 >>END_MODULE