FastQCFastQC Report
Wed 25 May 2016
SRR1781304_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781304_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147865
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2046513.840327325601054No Hit
CTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT70634.776654380685084No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT45693.0899807256619214No Hit
CTAAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT37442.5320393602272344No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36182.446826497142664No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG33152.241909850201197No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC16931.1449633111283941No Hit
TCATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG14520.9819768031650492No Hit
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT11470.7757075710952558No Hit
CAAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT10310.6972576336523181No Hit
CAAAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT8950.605281844926115No Hit
CATAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT8890.6012240895411355No Hit
TCAAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG5860.39630744259966866No Hit
TCTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG5080.3435566225949346No Hit
CTCATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC4230.2860717546410577No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC3660.24752307848375207No Hit
CTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT2950.19950630642816083No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTAGAAATCTCGTATGCCGT2790.1886856254015487No Hit
CTCTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2700.1825989923240794No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT2690.18192269975991615TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTACATGAAATCTCGTATGCCGT2390.16163392283501843No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT2370.16028133770669192No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC2290.15487099719338587TruSeq Adapter, Index 12 (95% over 21bp)
CTAAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT2020.13661109796097792No Hit
CTTATACACATCTCCGCGCCCACGAGACTACTTGAAATCTCGTATGCCGT1800.12173266154938626No Hit
CTCAAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC1760.11902749129273323No Hit
CTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT1760.11902749129273323No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT1680.11361715077942718No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC1480.10009129949616204TruSeq Adapter, Index 12 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT1480.10009129949616204No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTGC207.8506855E-570.09891551
TACAGCT301.0081931E-770.09891552
CAACTGC252.8051127E-670.09891551
GTCAACT252.8051127E-670.09891549
GACGTAT207.8506855E-570.09891546
TACTGCA207.8506855E-570.09891552
TATGCTA207.8638746E-570.0751853
ACTGCAT252.8107643E-670.0751753
TATGCCA150.00221162170.0751743
AATGCCC150.00221162170.0751743
AATGAAG252.8107643E-670.0751743
CCATGCT150.002220549470.0040641
ATGCTAG252.827781E-670.0040654
CGAAAGA150.002220549470.0040641
TAAAATC207.9168065E-569.98038533
TCGAAAG353.6925485E-969.98038540
TTGAGAT252.8334725E-669.98038532
CATTATA252.8334725E-669.9803852
TAACCGA353.6925485E-969.98038511
TGCTTTA207.9168065E-569.98038555
Produced by FastQC (version 0.11.5)