FastQCFastQC Report
Wed 25 May 2016
SRR1781303_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781303_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243667
Sequences flagged as poor quality0
Sequence length76
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7489030.734568078566237No Hit
CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG19870.8154571607973177No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7830.32134018968510303No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6430.26388472792786877No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3970.1629272736973No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3000.12311884662264483No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2520.10341983116302167No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAAGC150.002222794670.0000124
CCGTTTT150.002222794670.0000124
CCCGTAA252.3872862E-456.00000437
TTGCATA252.3872862E-456.00000431
ATTGCAT252.3872862E-456.00000430
AGATACG200.00694549752.56
TAGAACG200.00694549752.54
TTCGGTA200.00694549752.568
CCAACTT200.00694549752.523
ATACGTC200.00694549752.58
CGTTTTC200.00694549752.525
CGTATCA3350.050.14925456
CCGTATC3350.050.14925455
GCCGTAT3400.049.41176654
TCTCGGT3800.048.8157943
TTGTGCG651.8371793E-1048.4615362
TCGGTGG4000.048.12500445
GTATCAT3600.047.63889357
CGCCGTA3550.047.32394453
CTTGTAC305.8702345E-446.6666681