FastQCFastQC Report
Wed 25 May 2016
SRR1781303_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781303_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243667
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT2440310.01489738044134TruSeq Adapter, Index 5 (95% over 23bp)
CTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT64562.649517579319317TruSeq Adapter, Index 5 (95% over 23bp)
CTTAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT42441.7417212835550158RNA PCR Primer, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG38211.5681237098170864TruSeq Adapter, Index 5 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36571.5008187403300406No Hit
CTAAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT27761.1392597274148737No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC19490.7998621068917827RNA PCR Primer, Index 5 (95% over 21bp)
TCATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG12570.5158679673488819TruSeq Adapter, Index 5 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT12400.5088912327069319TruSeq Adapter, Index 5 (95% over 23bp)
CAAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT9610.3943907053478723TruSeq Adapter, Index 5 (95% over 23bp)
CATAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT7480.30697632424579446No Hit
CAAAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT7070.29015008187403296RNA PCR Primer, Index 5 (95% over 23bp)
TCAAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG4790.19657975844082293TruSeq Adapter, Index 5 (95% over 22bp)
TCTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG4530.1859094584001937TruSeq Adapter, Index 5 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC4340.1781119314474262RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACACAAAGTGATCTCGTATGCCGT4340.1781119314474262TruSeq Adapter, Index 5 (95% over 23bp)
CTCATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC3820.15677133136616778No Hit
CTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT3330.13666191975113576No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT3110.12763320433214181No Hit
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT3020.12393963893346246TruSeq Adapter, Index 5 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACAAAAAGTGATCTCGTATGCCGT2770.11367973504824205No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGAGATCTCGTATGCCGT2630.10793418887251865No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAAT207.871575E-570.07973547
ATTAAGC252.8254926E-670.0365651
TTAAACG150.002223621869.9934340
ACTCTCG150.00222543369.97906511
CCATAGG150.00222543369.9790651
CTAAAAC10000.065.430431
ATCGTAA750.065.313839
TAAAACA10350.064.570062
AGGGGGG32050.064.3153470
AACACAT25000.062.5612875
CGTCTAA401.053013E-861.31976748
AAACACA26350.061.08234
CTTAAAC8150.060.534041
AAAACAC15150.060.509953
TATGCTA352.9470903E-760.05600753
CGAATGA700.060.0436741
TTAAACA8500.059.276392
TAAACAC11800.059.0077743
CCGTATT2100.058.3997847
TAAGCCG3050.057.41880843
Produced by FastQC (version 0.11.5)