FastQCFastQC Report
Wed 25 May 2016
SRR1781302_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781302_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177234
Sequences flagged as poor quality0
Sequence length76
%GC90

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10681660.26834580272408No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12120.6838417008023292No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7540.42542627261135No Hit
CTTATACACATCTGACGCTGCCGACGAACTTCTAGGTGTAGATCTCGGTG6890.3887515939379577No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4690.2646219122741686No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4180.23584639516119932No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3110.17547423180653826No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2630.14839139217080244No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2190.12356545583804461No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2180.12300123001230012No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACAT150.002222040269.99802462
CGTGTGA150.002222040269.9980249
AAGTGTA150.002222040269.99802434
TTGTGCG453.8198777E-1062.2204672
AGGTCTC252.3858355E-455.9984240
TAGGTCT252.3858355E-455.9984239
GACGAAA200.006935413552.51333623
CTTGTGC608.367351E-1152.498521
TTATGCA200.006943160752.4985162
CACATGG200.006943160752.4985167
AGGTGTC200.006943160752.49851640
CTTGGGC200.006943160752.4985161
TCTCTGT200.006943160752.49851639
TTGGACG200.006943160752.4985162
TGTGCGC552.2682798E-950.9076543
TTGGGCG352.0806137E-549.998592
TTGTGGG352.0806137E-549.998592
GCGTCTG651.8371793E-1048.4601758
TCTGGCG1100.047.72592511
GTAGGTC305.8666925E-446.6653538
Produced by FastQC (version 0.11.5)