FastQCFastQC Report
Wed 25 May 2016
SRR1781302_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781302_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177234
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT7117840.16046582484174No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG94325.321777988422086No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC66043.7261473532166516No Hit
CATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT14510.8186916731552637No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13730.774682058747193No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC13140.7413927350282676No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT12800.7222090569529548No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT10980.6195199566674566No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT10520.5935655686832098No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGACGT9040.5100601464730243No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT8640.48749111344324453RNA PCR Primer, Index 48 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCAGT7730.4361465633004954No Hit
CTTATACACATCTCCGCGCCCACGAGACTTTCGTCAATCTCGTATGCCGT5980.3374070437952086No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC5830.32894365640904116No Hit
CTTATACACATCTCCGAGCCAACGAGACTTTCGTCAATCTCGTATGCCGT5300.2990396876445829No Hit
CTTATACACATCTCCGAGACCACGAGACTTTCGTCAATCTCGTATGCCGT3830.21609849126014197No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG3500.1974790390105736No Hit
TCATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG3390.19127255492738413No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG3370.19014410327589515No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC3270.18450184501845018RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTATCGTCAATCTCGTATGCCGT3000.1692677477233488No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC2980.16813929607185982No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2880.16249703781441485No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCTATCTCGTATGCCGT2860.16136858616292585No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGA2610.14726294051931346No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG2520.14218490808761297No Hit
CTTATACACATCTCCGAGCACACGAGACTTTCGTCAATCTCGTATGCCGT2360.13315729487570105No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCAG2330.13146461739846757No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGACAATCTCGTATGCCGT2280.1286434882697451No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCACTCTCGTATGCCGT2220.1252581333152781No Hit
CTTATACACATCTCAGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT2170.12243700418655562No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC2100.11848742340634416No Hit
CTTATACAAATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT2040.11510206845187718No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGACG1940.10945981019443221No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT1870.10551022941422074RNA PCR Primer, Index 48 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTATT207.8411445E-570.1238747
ACGTAAT150.002206259970.1238747
CCGTAAT150.002206259970.1238747
GCAGTAT207.8411445E-570.1238746
GACGTAA150.002206259970.1238746
GCCGTAA207.8411445E-570.1238746
TGCAGTA252.8014838E-670.1238745
CGACTTA301.0068652E-770.1238748
CCGAATT150.002206259970.1238747
TAAGACG252.8061968E-670.1040443
AACCCGA150.002208736370.1040444
CGTAAGA301.0088479E-770.1040441
ATTATGC207.885197E-570.0446451
CTTGCAA207.885197E-570.0446457
ATTCTGA207.885197E-570.0446451
ACTTCAG150.002216178570.0446350
ACTTATG252.8203722E-670.0446350
TATTATG150.002216178570.0446350
CTTGAAT150.002216178570.0446357
GCATCAA150.002216178570.0446356
Produced by FastQC (version 0.11.5)