FastQCFastQC Report
Wed 25 May 2016
SRR1781301_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781301_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280392
Sequences flagged as poor quality0
Sequence length76
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9193932.78945191018289No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10440.3723358726354532No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7200.25678336043824357No Hit
CTTATACACATCTGACGCTGCCGACGAACTTCTAGGTGTAGATCTCGGTG5900.21041969813689407No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4580.16334274872321608No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3840.13695112556706326No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGCT150.002223145770.014
AAAGTTC150.002223145770.014
GAGGTTA150.002223145770.058
TGAAGTA252.387909E-456.00000463
GTTAGAT252.387909E-456.0000041
AAGTCTT252.387909E-456.00000435
TCATTAA1750.054.060
AGATATG200.00694658452.4999964
AGATAGG200.00694658452.49999632
ATGCTTC200.00694658452.49999658
CTTGATA200.00694658452.49999640
TACTCAA200.00694658452.49999637
AAGGGAT200.00694658452.49999630
TATGTCC200.00694658452.4999964
ATGCACA200.00694658452.49999664
CATTAAA1900.049.7368461
ATCATTA1900.047.89473359
CTTGTGC604.9185473E-946.6666641
AAAGTCT305.8717595E-446.66666434
GTCTTTA305.8717595E-446.66666437
Produced by FastQC (version 0.11.5)