FastQCFastQC Report
Wed 25 May 2016
SRR1781301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781301_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280392
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT5992921.373291677365973No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG75732.700861650831693No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC61452.191574652629176No Hit
CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT12950.46185340523267426No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC11500.41014008958886133No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11060.3944477731176353No Hit
CTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT10980.39159462466832146No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT10380.37019601129846785No Hit
CTTAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT9880.35236383349025646No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGT9860.35165054637792803No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT7320.2610630831122143TruSeq Adapter, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT7250.25856657821906476No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC5250.1872378669862193No Hit
CTTATACACATCTCCGCGCCCACGAGACGAGCACGTATCTCGTATGCCGT4680.16690918428485835No Hit
CTTATACACATCTCCGAGCCAACGAGACGAGCACGTATCTCGTATGCCGT4020.14337070957801934No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGAATCTCGTATGCCGT3660.13053154155610716No Hit
CTTATACACATCTCCGAGACCACGAGACGAGCACGTATCTCGTATGCCGT3560.1269651059944649No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAACGTATCTCGTATGCCGT3280.11697908642186654No Hit
TCATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG3250.11590915575337385No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCAAGTATCTCGTATGCCGT3220.11483922508488116No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC2960.10556649262461126No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTAT150.00221506470.06428553
AGATAGC150.002216633470.0517850
GATTACT207.917176E-570.00178557
GTTGACA150.002226067769.97681416
CACGGTA150.002226067769.97681414
TCTTAAT252.840974E-669.9768141
GCAAACG353.7089194E-969.97681418
AATATCG150.002226067769.97681436
TATCGAG150.002226067769.97681436
AGGGGGG55800.065.71370
TAAGCCG1800.062.30161743
GCCGTAA401.051194E-861.3391146
ACGAATC2300.060.84940333
TCTTTAT2250.060.6465721
GAATCTC2200.060.4345235
AATCTCG2200.060.4345236
CGAATCT2150.060.2126134
TCGAATG1300.059.24284740
CTAAAAC1600.059.042941
ATTCCGT308.2918505E-658.41820544
Produced by FastQC (version 0.11.5)