FastQCFastQC Report
Wed 25 May 2016
SRR1781300_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781300_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278183
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7505726.981159883961276No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9370.3368286343881545No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7360.2645740393913359No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4150.14918237275462554No Hit
CTTATACACATCTGACGCTGCCGACGAACTTCTAGGTGTAGATCTCGGTG3800.13660072685965713No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3010.1082021546967284No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT150.002223126670.0000124
TATGCGC207.918071E-570.000013
TCGGTAT150.002223126670.0000136
GTCCATA252.3878763E-456.00000429
AAACTCG200.00694652552.50000411
GGATATA200.00694652552.50000458
TCGTACG200.00694652552.5000041
TATAAAG200.00694652552.50000433
GCCGTAT1200.049.58333654
CGTATCA1400.047.50000456
TTGGTCC305.8716774E-446.66666855
CCGTATC1300.045.76923455
TGGTCGC1600.041.56250449
GTATCAT1450.041.0344857
GTAGGTC350.001254128540.00000438
ATGTCGG350.001254128540.00000442
CGGATAC350.001254128540.0000043
ATTGCGC350.001254128540.0000049
GTTATTG350.001254128540.00000450
TCTGGCG1250.039.211