SRR1781300_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1781300_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	278183
Sequences flagged as poor quality	0
Sequence length	76
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	48662	17.492801501170092	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	6323	2.272964199825295	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	4337	1.559045664185087	No Hit
CATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	1288	0.4630045689348379	No Hit
CTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	1065	0.38284151080403905	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	984	0.35372398744711214	No Hit
CTTAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	879	0.3159790497622069	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	833	0.2994431723002484	No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT	791	0.2843451972262863	RNA PCR Primer, Index 47 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGACGT	695	0.2498355399143729	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCAGT	621	0.22323434573643966	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGCTCTCGTATGCCGT	527	0.1894436396185245	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT	474	0.1703914329775723	No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	446	0.16032611626159757	No Hit
CTTATACACATCTCCGAGCCAACGAGACAATGGTAGATCTCGTATGCCGT	403	0.14486866559063638	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGAAGATCTCGTATGCCGT	380	0.13660072685965713	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGGTAGATCTCGTATGCCGT	324	0.11647009342770766	No Hit
CTTATACACATCTCCGAGACCACGAGACAATGGTAGATCTCGTATGCCGT	318	0.11431323984571308	No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGGTAGATCTCGTATGCCGT	306	0.1099995326817239	No Hit
CTTATACACATCTCCGCGCCCACGAGACAATGGTAGATCTCGTATGCCGT	296	0.10640477671173293	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGGG	4765	0.0	64.56006	70
TCGAATG	165	0.0	59.389824	40
AATGACG	30	8.357998E-6	58.33977	43
ACTATCG	25	2.3886946E-4	55.99612	24
ATACGAG	25	2.3886946E-4	55.99612	12
ACGTATG	95	0.0	55.259327	40
ATCGTAT	205	0.0	54.63036	39
ATATCGT	205	0.0	54.63036	37
TATCGTA	220	0.0	54.08716	38
CTTGAAA	8520	0.0	53.039898	57
TTGAAAA	8640	0.0	53.02294	58
CCGTCTT	7950	0.0	53.021675	47
TATGCCG	8345	0.0	52.97769	43
CGTATGC	8580	0.0	52.954567	41
TGAAAAA	9000	0.0	52.92411	59
GTATGCC	8380	0.0	52.881733	42
TCAGCTT	245	0.0	52.872482	53
TCGTATG	8820	0.0	52.774117	40
GCTTGAA	8485	0.0	52.763634	56
CTGCTTG	8155	0.0	52.75261	54

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content