FastQCFastQC Report
Wed 25 May 2016
SRR1781299_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781299_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337068
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8922726.47151316648273No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10320.3061696749617288No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7390.21924359476426122No Hit
CTTATACACATCTGACGCTGCCGACGAACTTCTAGGTGTAGATCTCGGTG5560.16495187914604767No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4630.13736100727449654No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4110.12193385310975827No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGTC252.3886052E-455.99999641
TAACGTT200.00694779652.544
ATAACGT200.00694779652.543
ATACGAA200.00694779652.537
GATAGGT305.873458E-446.6666687
TGGTACT305.873458E-446.6666682
TGGATTC350.001254506640.028
TTTGAGC350.001254506640.03
GCCGTAT1250.039.254
GTATCAT1750.038.057
TAGATCT1750.038.039
CGTATCA1500.037.33333656
GCGTGTG1151.8189894E-1236.521748
TGGCGCT2150.035.81395313
GAAGCAC601.21400135E-535.0000048
ATAGACG400.00241701635.04
CGATTCT400.00241701635.048
TCGATTC400.00241701635.047
CGCGTCT806.2287654E-835.07
ATCATTA1800.034.99999659