FastQCFastQC Report
Wed 25 May 2016
SRR1781299_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781299_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337068
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT5878417.43980443115336No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG75762.2476176913857144No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC60691.800526896649934No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT12940.3838987978686793No Hit
CTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT11640.34533091245683367No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11320.335837279124687No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC10570.31358657600246836No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT9310.27620539475714095No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT8160.24208764996973905No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT7830.23229734059596283Illumina PCR Primer Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT6850.2032230885162638No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGT6440.19105937080945093No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT5940.1762255687279718No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC5170.15338151352249396No Hit
CTTATACACATCTCCGCGCCCACGAGACGTTAACAGATCTCGTATGCCGT4630.13736100727449654No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACAGATCTCGTATGCCGT3760.1115501916527229No Hit
CTTATACACATCTCCGAGACCACGAGACGTTAACAGATCTCGTATGCCGT3470.102946586445465No Hit
CTTATACACATCTCCGAGCCAACGAGACGTTAACAGATCTCGTATGCCGT3400.10086985415405794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT750.070.059746
TATTGTA150.002225181869.98694610
AGGGGGG58650.064.3784470
CCGTATT900.062.27528447
CACGTAT850.061.7531939
ATTCCGT308.339786E-658.36574644
TCGTATC308.376555E-658.32245640
CGTTAAA1150.057.81530428
AGCTAGA850.057.6705355
ACGTATG1050.056.656140
TAAGACG252.382056E-456.03111343
CTCGAAA509.804353E-1055.98955539
TGCCGTA1150.054.82118245
TCTTTAT2050.054.623961
TCGTATT452.699926E-854.4342940
GTATTCT1050.053.35504549
GACGATA3150.053.3233926
TCGAATG1850.052.96309740
TCGAAAG608.367351E-1152.4902140
CTATCGA200.006952923352.49020837