FastQCFastQC Report
Wed 25 May 2016
SRR1781298_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781298_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320205
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8534126.65198856982246No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8830.27576084071141926No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6730.21017785481176118No Hit
CTTATACACATCTGACGCTGCCGACGAACTTCTAGGTGTAGATCTCGGTG5260.1642697646820006No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4240.13241517153073812No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTA150.002223434670.000015
TATCCGC150.002223434670.0000138
AGGTACG200.006947479752.50000426
ACAATCG200.006947479752.50000437
CAATCGA200.006947479752.50000438
TTACACG200.006947479752.50000451
TCGGTAT200.006947479752.50000414
CGTATCA1150.048.69565256
GCCGTAT1150.048.69565254
CACCTTA305.873015E-446.66666828
TAGTCCC305.873015E-446.6666684
CCATTCG305.873015E-446.66666867
TATCATT1400.045.00000458
CGCGTCT703.8380676E-1045.0000047
GTATCAT1250.044.857
TCATTAA1550.042.9032260
TGTGCGC757.530616E-1042.03
CCGTATC1350.041.4814855
ATCATTA1650.040.30303259
CAACGAA350.001254412640.00000470
Produced by FastQC (version 0.11.5)