FastQCFastQC Report
Wed 25 May 2016
SRR1781298_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781298_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320205
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT5674617.721771989819022No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG72532.265111413001046No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC58501.8269546072047596No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT11320.35352352399244236No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9640.30105713527271594No Hit
CTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT9610.30012023547414934No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC9400.2935619368841835No Hit
CTTAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT8270.2582720444715104No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT7430.2320388501116472No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT6970.21767305320029356RNA PCR Primer, Index 42 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACGT6090.1901906591090083No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGT5430.16957886354054436No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC5300.16551896441342265RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT5190.16208366515201197No Hit
CTTATACACATCTCCGCGCCCACGAGACATCACTCGATCTCGTATGCCGT3660.11430177542511828No Hit
CTTATACACATCTCCGAGACCACGAGACATCACTCGATCTCGTATGCCGT3280.10243437797660873No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAA150.002226367869.9767424
AGGGGGG53200.065.9649170
TAACTCG700.064.9986930
CATAACT850.061.76346228
ACGTATG900.058.35951240
AATGCCG1050.056.72755443
CACGTAT750.056.02512739
TAACGTA252.3868165E-456.0076238
ACTTCGT200.006930874752.5317754
AATCGAT608.367351E-1152.49894732
TACGAGA200.006956618752.4825513
TTTTCGA200.006956618752.4825522
GCTTGAA95750.052.28682756
ATCTCGT93850.052.28451537
CGTATGA2750.052.22971741
TATGCCG94400.052.14813643
CTTGAAA96100.052.11654357
CGTATGC96050.052.08316441
TCGTATG97850.052.03151340
CTCGTAT95950.052.0035239