FastQCFastQC Report
Wed 25 May 2016
SRR1781297_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781297_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303592
Sequences flagged as poor quality0
Sequence length76
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11592538.18447126406493No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12150.4002081741284355No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10550.34750586313209836No Hit
CTTATACACATCTGACGCTGCCGACGAACTTCTAGGTGTAGATCTCGGTG8310.2737226277372263No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6020.19829244512371866No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4320.1422962396901104No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4260.14031990302774777No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3530.11627447363566892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCA308.365307E-658.3333322
TTGTGCG750.056.02
GTGTTAT252.3882254E-455.99999649
CTCCGTA252.3882254E-455.99999626
TGCCTCG252.3882254E-455.99999642
CGTATCA1650.053.03030456
TTCTATA200.006947133752.545
AAGTATC200.006947133752.545
GGTACCA200.006947133752.543
ACGTTCA200.006947133752.521
TAGACCA200.006947133752.510
GTGGCTA200.006947133752.513
CATTCGG200.006947133752.551
TACTGGC200.006947133752.570
ACAGCGT200.006947133752.58
TGCTAAC200.006947133752.52
ATTATGC200.006947133752.53
TTCGCCT200.006947133752.556
GCCGTAT1600.052.554
CCGTATC1750.050.055