FastQCFastQC Report
Wed 25 May 2016
SRR1781297_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781297_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303592
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT7678725.292827215473395No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG102533.377229966534032No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC69092.275751666710585No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16080.5296582255131888No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT15850.5220822683074653No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC14530.47860286173548705No Hit
CTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT14410.4746501884107618No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT12420.40910168910906747No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT11500.3787978602861735RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT11330.37319823974281274No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT9880.32543677040238217No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT9510.31324936098447914No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC7020.23123138949642943No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT6190.20389206566707951No Hit
CTTATACACATCTCCGCGCCCACGAGACATAAGTTAATCTCGTATGCCGT5470.18017602571872776No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC5180.17062373185064164No Hit
CTTATACACATCTCCGAGCCAACGAGACATAAGTTAATCTCGTATGCCGT4990.1643653324198266No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG4770.15711876465783026No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTAAATCTCGTATGCCGT4730.15580120688292182No Hit
CTTATACACATCTCCGAGACCACGAGACATAAGTTAATCTCGTATGCCGT4480.1475664707897441No Hit
TCATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG4150.13669661914674958No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC3240.1067221797675828No Hit
CCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG3130.10309889588658463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC301.01883415E-770.0288541
AATCCGA150.002225502969.98270411
TTAATCG207.928933E-569.98270434
TGAAACG150.002225502969.98270414
ACGTATC150.002225502969.98270440
AATCGAC150.002225502969.98270436
AGGGGGG76700.065.7399570
TCGTATC501.4551915E-1162.98443240
AGTTTAT950.062.61610832
CGTCTTA1250.061.6660448
TCTTTAT2050.061.4482271
ATATCGA401.0657459E-861.23486737
ATATACG352.973702E-759.98517610
CTAAAAC1750.059.9851761
TATACGA352.973702E-759.98517611
GTATCCC308.328594E-658.37661442
GTCTACT2100.058.3669949
CACGATA308.377565E-658.3189221
AATCGTA308.377565E-658.3189238
TAACTTA308.377565E-658.3189230
Produced by FastQC (version 0.11.5)