FastQCFastQC Report
Wed 25 May 2016
SRR1781296_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781296_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297659
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9264631.12487779640461No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10230.343681864146557No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7070.23752011529972217No Hit
CTTATACACATCTGACGCTGCCGACGAACTTCTAGGTGTAGATCTCGGTG4350.14614038211510486No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4300.1444606076080347No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3690.1239673586217786No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAG150.002223280570.03
ACGGGTT150.002223280570.05
GCCGTAT850.053.52941554
GTCGTAC200.006947002452.59
AAATTCG200.006947002452.552
TCTTTAC200.006947002452.530
TAGTGTC200.006947002452.544
CAGTAGT200.006947002452.528
GACCTTA200.006947002452.521
AATACGG200.006947002452.55
TTGTGCG750.051.3333322
GCTCTAG352.0837138E-550.0000041
GTCTGGC950.047.8947410
TAAGGCT305.8723456E-446.6666644
GGACAAT305.8723456E-446.6666646
CGTATCA1150.045.65217256
CCGTATC1000.045.50000455
CTTGTGC703.8198777E-1045.0000041
TGTGCGC802.910383E-1143.753
ATCATTA1250.042.00000459
Produced by FastQC (version 0.11.5)