SRR1781296_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1781296_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	297659
Sequences flagged as poor quality	0
Sequence length	76
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	61533	20.672312948709763	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	8166	2.7434077249470032	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	5347	1.7963508578608407	No Hit
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	1460	0.4904941560644899	No Hit
CTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	1148	0.38567622682331126	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	1082	0.363503203329985	No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	1056	0.3547683758932201	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	916	0.3077346896952553	No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT	840	0.2822021171877887	Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACGT	817	0.27447515445526594	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAGT	678	0.22777742315871516	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCGT	662	0.22240214473609063	No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	577	0.1938459781158977	No Hit
CTTATACACATCTCCGCGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	522	0.17536845853812583	No Hit
CTTATACACATCTCCGAGCCAACGAGACTTGCTAAGATCTCGTATGCCGT	409	0.13740555467833998	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT	386	0.12967859194581718	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	372	0.12497522332602072	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTTAGATCTCGTATGCCGT	364	0.12228758411470846	No Hit
TCATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	338	0.11355275667794355	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCG	322	0.108177478255319	No Hit
CTTATACACATCTCCGAGACCACGAGACTTGCTAAGATCTCGTATGCCGT	320	0.10750556845249093	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCTAAGATCTCGTATGCCGT	317	0.10649770374824884	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	317	0.10649770374824884	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGGG	5855	0.0	64.78688	70
TAAGACG	45	3.8198777E-10	62.26257	43
ATATCGT	205	0.0	59.744625	37
CAAAGAT	195	0.0	57.42487	32
TCACGTA	110	0.0	57.27135	38
CTTTATA	190	0.0	57.094296	2
TAAGCCG	130	0.0	56.57513	43
CACGTAT	120	0.0	55.415337	39
AAGATAT	275	0.0	54.716766	34
TAAGATA	275	0.0	54.716766	33
GATATCG	245	0.0	54.275295	36
ATCGTAT	240	0.0	53.95704	39
TATCGTA	235	0.0	53.615738	38
TCGTAAG	190	0.0	53.419773	40
CCGTCTT	9935	0.0	53.23027	47
CGTCTTC	10135	0.0	53.21653	48
GCCGTCT	10085	0.0	53.20255	46
CGTATGC	10590	0.0	53.037735	41
TATGCCG	10355	0.0	53.032917	43
GTATGCC	10380	0.0	53.03124	42

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content