FastQCFastQC Report
Wed 25 May 2016
SRR1781295_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781295_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319464
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9333629.216437532867552No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10150.3177196804647785No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6670.20878721859114013No Hit
CTTATACACATCTGACGCTGCCGACGAACTTCTAGGTGTAGATCTCGGTG4800.150251671549846No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4470.13992186913079407No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3930.12301855608143641No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCT150.002223567869.998917
GCCGTAT1250.061.59903754
CCGTATC1250.058.7990855
TGTGCGC800.056.874113
CGTATCA1350.054.4435956
TATAGAC200.006947895552.499183
TGCGTCG352.0823245E-550.00704210
GCGTGTG1300.048.4683658
GTATCAT1550.045.16058357
GTGTGCG802.910383E-1143.7493132
AAAGGTG404.587746E-543.74931344
ATCATTA1550.042.90255459
TCATTAA1600.041.56184860
ATCTCGG1700.041.17582742
CGTGTGG1550.040.6508879
TGTGGCG1300.040.39030511
TTGTGCG701.9210347E-839.9993742
TAGATCT1850.039.72910739
TCTCGGT1950.039.48656543
TGGTCGC2250.038.8882849
Produced by FastQC (version 0.11.5)