FastQCFastQC Report
Wed 25 May 2016
SRR1781295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781295_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319464
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT5124316.040305010893245No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG63581.9902085994040017No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC46341.4505546790874715No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT23060.7218340720707184No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT19710.6169709263015551No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10160.3180327047805073No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTAAGCCGT8860.27733954373575737No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC8520.26669671700097664No Hit
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT7920.24791525805724587No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCAGGATCTCGTATGCCGT7170.22443843437758246No Hit
CTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT6560.20534395111812287No Hit
CTTAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT5700.17842385996544213No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCAGT5590.1749805924924248No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGA5210.1630856684947287No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGACGGATCTCGTATGCCGT4390.1374176746049633No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT4340.13585255302631907RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT4300.1346004557634037No Hit
CTTATACACATCTCCGCGCCCACGAGACATTGCCGGATCTCGTATGCCGT4060.1270878721859114No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCG3850.12051436155560563No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATTCCGT3770.11801016702977488No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC3580.1120627050309268No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCGCGTATGCCGT3560.1114366563994691No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGACGT3270.1023589512433326No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGAATGCCGT3230.1011068539804172No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAA207.9157464E-570.00735539
TATACCG301.02118065E-770.00735543
ATATCGA207.921887E-569.996437
AGGGGGG44100.064.6099670
TGACGTA1500.058.33033445
TAAGACG750.056.0058943
TTAGCCG252.387175E-456.00588643
TATCGTA1750.056.00588638
CGGATAA252.3890224E-455.99711634
ATTGACG2000.055.99711629
GCCGTAT6200.055.9017246
CCGTATT6350.055.6838647
TGCCGTA8900.055.4465945
TTATGCT6600.055.1659552
ATCGTAT1650.055.15731439
TAAACAC5250.054.6638573
ATTAAGC900.054.45868751
TATGCCG86850.054.20834443
GTATGCC87750.054.13104242
CCGTAAT2850.054.0492347
Produced by FastQC (version 0.11.5)