FastQCFastQC Report
Wed 25 May 2016
SRR1781294_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781294_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482544
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10401021.55451109121655No Hit
CTTATACACATCTGACGCTGCCGACGAACTTCTAGGTGTAGATCTCGGTG14860.3079511920156504No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10910.22609337179614708No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10200.21137968765542625No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5110.1058970788156106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA2650.051.50790856
GCCGTAT2650.050.1871954
CCGTATC2800.047.49859655
GTATCAT3200.041.56557557
TTGTGCG855.638867E-1141.179522
TGGTCGC3900.040.3834249
CGCCGTA3300.040.30183853
TTTGCGG350.001255227539.99881744
GTAGATC3250.039.84910638
TCTCGGT3450.039.56404543
TATCATT3550.038.4535558
TAGATCT3500.037.0027439
ATCATTA3600.036.94717859
GTGGTCG4350.036.20582648
TCGGTGG4350.035.40125345
CGCGTCT806.240043E-834.9989627
CGGTGGT4650.034.6226346
TGTAGAT4000.034.12752537
TCATTAA4200.033.33580460
AGATCTC3900.033.2075940
Produced by FastQC (version 0.11.5)