FastQCFastQC Report
Wed 25 May 2016
SRR1781294_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781294_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482544
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT6770214.030223150634969No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG87781.8191087237640506No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC72941.5115720017241951No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26270.5444063132066713No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT17250.3574803541231473No Hit
CATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT12860.26650419443615503No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC12140.2515832753075367No Hit
CTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT12090.25054710036804934No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT10650.22070526211081268No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCAGT9140.1894127789382937No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT8120.1682748101727511Illumina PCR Primer Index 10 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC6240.12931463244802546Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTAGTATGCCGT6040.12516993269007592No Hit
CTTATACACATCTCCGCGCCCACGAGACAGTAGATTATCTCGTATGCCGT5430.11252859842832985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG61800.063.81108570
TAACTCG1250.061.5847736
GACGTAT352.9476723E-760.07232346
TATGACG6050.055.00779343
ATATCGT1600.054.6739837
AATCTCG2650.054.13755436
ATGACGT6300.053.94264644
TGACGTA653.6379788E-1253.91105745
TAAGCCG2600.053.8942743
ACGTATG1300.053.8384240
GTATGAC6250.053.80804442
AAGCCGT2800.053.8036244
CGTATGA5750.053.5852941
TATGCCG111850.052.7113543
CTGCTTG99650.052.70960654
GTCTTCT99450.052.56913849
CCGTCTT101500.052.54601747
TGCTTGA102800.052.5428455
GCTTGAA109850.052.5113156
TAAAACG200.00695640252.48702236