FastQCFastQC Report
Wed 25 May 2016
SRR1781293_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781293_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352698
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9341226.484981485576895No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10330.29288513118872234No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6590.18684540314943662No Hit
CTTATACACATCTGACGCTGCCGACGAACTTCTAGGTGTAGATCTCGGTG5420.1536725470515852No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4610.13070672359922653No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4090.11596323200018147No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGT200.006948062752.49999623
GAATATG200.006948062752.4999961
AAGCGTT200.006948062752.4999966
TAGTGCG200.006948062752.4999962
GCACTTA407.4694253E-752.4999961
TTGTGCG604.9240043E-946.6666682
CGTATCA1300.043.07692356
GCCGTAT1400.040.054
CTTGTGC654.8573384E-737.6923071
CGTGTGG1151.8189894E-1236.5217369
CGCGTGT601.2141605E-535.0000047
GTCTAGC501.7080846E-435.01
TGTAGGT708.677198E-735.037
ACGGCTA400.002417167934.99999656
TTTGCCC400.002417167934.99999627
GTATCAT1650.033.93939257
TGTGCGC851.0614531E-732.9411773
CCGTATC1600.032.81249655
TGGTCGC2250.032.66666849
GGTCCTA552.9892792E-431.818181
Produced by FastQC (version 0.11.5)