FastQCFastQC Report
Wed 25 May 2016
SRR1781293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352698
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT6140517.410078877680053No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG80262.275601222575688No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC64681.8338635319735297No Hit
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT12470.3535602696924848No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT11940.33853324940884266No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11470.32520740123278274No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC11290.3201038849100364No Hit
CTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT10350.2934521885579164No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT9670.27417223800531904No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT7570.21463121423994463No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT7450.2112288700247804RNA PCR Primer, Index 24 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC5660.16047723548191373No Hit
CTTATACACATCTCCGCGCCCACGAGACGTGATAGCATCTCGTATGCCGT4850.13751141202955502No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGAATCTCGTATGCCGT3960.11227735910042018No Hit
CTTATACACATCTCCGAGACCACGAGACGTGATAGCATCTCGTATGCCGT3640.10320444119331554No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT3580.10150326908573341No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG57850.066.4008270
TAAGACG509.658834E-1056.08637643
AATATCG252.394023E-455.97504836
ACTCGTA1350.054.427938
AACACAT6350.053.4407425
CAACTCG1250.053.17629236
CCGTCTT96350.052.79767247
GTCTTCT96100.052.6497349
GACGTAT200.00690195352.58844846
CGTCTAA200.00690195352.58844848
ACGTATT200.00690195352.58844847
TATGCCG101500.052.5291743
TCGTAAG2600.052.4989340
GCCGTCT97400.052.4804746
CCTTAGT200.006960318452.4766041
TAGGTTC200.006960318452.4766044
CGTTATA200.006960318452.47660428
GCACTAA200.006960318452.47660416
CGTCTTC98300.052.46361548
CTTCTGC95850.052.3483851
Produced by FastQC (version 0.11.5)