FastQCFastQC Report
Wed 25 May 2016
SRR1781292_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781292_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304882
Sequences flagged as poor quality0
Sequence length76
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10616934.822980694170205No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10750.3525954303632225No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9010.29552417000675674No Hit
CTTATACACATCTGACGCTGCCGACGAACTTCTAGGTGTAGATCTCGGTG7100.2328769819143144No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5040.1653098575842457No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3610.11840646545220775No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCTT150.002223332270.042
TTGCGCG252.3882413E-456.00000417
GTAGGCT200.006947163552.549
TGTGCGC800.052.53
ATACTTC200.006947163552.56
ACGCTTG200.006947163552.543
GGTTAGA200.006947163552.52
GTTATAG200.006947163552.51
GTATCAT1500.051.33333257
TTGGGCG552.2773747E-950.9090962
GCCGTAT1600.050.312554
CGTATCA1800.048.61110756
CCGTATC1750.048.055
GGTCCTA305.8725703E-446.6666641
CATTAAA1750.046.061
CGCCGTA1800.044.7222253
TTGTGCG551.3083627E-744.5454562
TCATTAA1650.044.54545660
GCGTGTG802.910383E-1143.758
ATCATTA1700.043.23529459
Produced by FastQC (version 0.11.5)