FastQCFastQC Report
Wed 25 May 2016
SRR1781292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304882
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT7033123.068269035233303No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG89842.946713810589015No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC68592.249722843591947No Hit
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT13900.45591409135337607No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13550.4444342401322479No Hit
CTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT12730.4175385886998904No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC12030.39457888625763404No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT11320.37129118806620265No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT11220.36801123057445173No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT9950.3263557704292152TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT7440.2440288373862675No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC5640.18498960253475116TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACTACTTGAAATCTCGTATGCCGT5620.18433361103640097No Hit
CTTATACACATCTCCGAGCCAACGAGACTACTTGAAATCTCGTATGCCGT4770.15645397235651826No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT4670.15317401486476737No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG4080.133822265663437No Hit
CTTATACACATCTCCGAGACCACGAGACTACTTGAAATCTCGTATGCCGT3980.1305423081716861No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTAGAAATCTCGTATGCCGT3680.12070243569643337No Hit
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC3310.10856659297695502RNA PCR Primer, Index 12 (95% over 21bp)
TCATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG3210.10528663548520412No Hit
CCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG3120.10233467374262828No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT150.002220397870.023358
AGGGGGG65600.065.6571270
AAACTCG1550.063.20536436
AACTCGT1250.061.58008237
GAATCTC401.0664735E-861.23019434
CGAGAAT401.0664735E-861.23019423
TAAGCTT700.060.0791453
CGTATTA308.300263E-658.4102748
CGTAATA308.300263E-658.4102748
GTCTTAA553.45608E-1157.3294349
TAAGCCG1650.057.31060843
AAAAGCG553.6379788E-1157.2542167
AGAATAC553.6379788E-1157.2542125
CACGTAT1050.056.64834639
ACTCGTA1300.056.5201838
TAAGACG252.3804493E-456.03703743
AGATCTT252.3920607E-455.98188813
ATCTAAT252.3920607E-455.9818881
CACGATA252.3920607E-455.98188821
TCTTTAT2000.055.9818881
Produced by FastQC (version 0.11.5)