FastQCFastQC Report
Wed 25 May 2016
SRR1781291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504232
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11383822.576512399054405No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11220.22251661933395736No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11080.21974011962747309No Hit
CTTATACACATCTGACGCTGCCGACGAACTTCTAGGTGTAGATCTCGGTG7540.14953434133494106No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5650.11205159529740279No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT150.002224265869.99930654
GTTATGC252.3886918E-456.0049971
TTTCGCG200.006947332552.5046844
GCCGTAT1650.050.9085954
CGTATCA1750.049.99950456
CCGTATC1700.049.41127455
GTATAAG1650.042.428031
TAGATCT2300.041.08654839
GTATCAT2100.038.33295457
ACGCAAC556.6921875E-638.1814415
ATCATTA2350.037.23367359
GTAGATC2600.036.3457938
CGCCGTA2650.035.66002353
TGTGCGC1400.035.0031243
TATAAGA1800.035.0031242
AGATCTC2950.034.4064440
TGTAGAT3250.033.38428537
ATAAGAG1800.033.0585063
CTAGACT851.0621625E-732.944124
TTGTGCG1600.032.815432
Produced by FastQC (version 0.11.5)