FastQCFastQC Report
Wed 25 May 2016
SRR1781291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781291_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504232
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT7070314.021918481968617TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG94521.874533944692126TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC68551.359493249139285RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT14800.29351568325691346No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14420.28597946976788463No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC13440.26654397182249445RNA PCR Primer, Index 5 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT13130.2603960081867077TruSeq Adapter, Index 5 (95% over 23bp)
CTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT11620.2304494756381983TruSeq Adapter, Index 5 (95% over 23bp)
CTTATACACATCTCCGCGCCCACGAGACACAAAGTGATCTCGTATGCCGT10980.21775690555141283TruSeq Adapter, Index 5 (95% over 23bp)
CTTAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT9880.19594155071475036RNA PCR Primer, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT9060.1796791952910565TruSeq Adapter, Index 5 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT8580.17015976772596741No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCACGTATGCCGT7750.15369909089466754No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCAGT7290.1445763061447905No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGCTCTCGTATGCCGT6320.1253391296070063No Hit
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC6220.12335591553094608TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTAGTATGCCGT5990.11879452315600755No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTAAGCCGT5480.1086801313681004No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAA207.929426E-569.99087538
AGGGGGG63300.063.96480670
ATCGTAT4200.055.82605439
TATCGTA4150.055.65539638
ATATCGT4050.055.30143437
CGAATGC2350.053.62598841
CACGAAT1050.053.3263839
AGTGATA5600.053.11807633
CGTCTTA4850.052.71013648
ATGCGTT200.006945191452.50877856
TCTGCTT107700.052.0803253
TATGCCG119300.052.0401143
GCTTGAA118550.052.02894656
CTGCTTG108100.052.00684454
CCGTCTT111000.051.96173547
TGCTTGA112350.051.9401555
TTGAAAA121800.051.87648858
ATGCCGT119650.051.8586244
GCCGTCT112050.051.81860446
TCGAATG2500.051.7983840
Produced by FastQC (version 0.11.5)