FastQCFastQC Report
Wed 25 May 2016
SRR1781290_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781290_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223078
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5191123.270335936309273No Hit
CTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTG28741.288338608020513No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6820.3057226620285281No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3390.15196478361828597No Hit
TCTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGT2980.13358556200073515No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2420.10848223491334869No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCC252.3844677E-456.01130362
CGGAAGT200.00694534552.49882534
CATTGGT200.00694534552.49882515
GTTAATA200.00694534552.49882524
GGTATAG200.00694534552.4988251
ACTGTCG200.00694534552.4988258
CAACTTC200.00694534552.4988256
TGTAATG200.00694534552.4988253
CGTATCA5150.046.89215556
GTTGTGC305.8698107E-446.665621
CGCAGTA305.8698107E-446.6656255
GCCGTAT5100.046.6656254
CCGTATC5150.046.2125655
GTATCAT5250.045.34248457
TCTCGGT5450.044.9531243
TATCATT5250.044.67568258
GTAGATC5600.044.37400438
ATCATTA5400.044.0829759
CGCCGTA5400.044.07308653
TGTAGAT5850.043.67423237
Produced by FastQC (version 0.11.5)