FastQCFastQC Report
Wed 25 May 2016
SRR1781290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781290_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223078
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT84063.768188705295906No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT83493.74263710451053No Hit
CAAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT51092.2902303230260266No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47982.1508172029514343No Hit
CATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT32671.4645101713302073No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG25211.1300979926303805No Hit
TCATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG22170.993822788441711No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC17260.7737204027290903No Hit
CACTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC6180.2770331453572293No Hit
CTAATACCCATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT5840.2617918396256018No Hit
CAAATACCCATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT5070.2272747648804454No Hit
CAAAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT4210.1887232268533876No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC3700.16586126825594635No Hit
CTTATACCCATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT3530.1582406153901326No Hit
CTAAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT3300.1479303203363846No Hit
TCATTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC3240.14524067814845032No Hit
CTAATACACATCTCCGCGCCCACGAGACTTTCGTCAATCTCGTATGCCGT2930.13134419351078996No Hit
AAAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT2750.12327526694698716No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2260.10130985574552398No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAAAG150.002221559270.0078540
TAACGTA207.910242E-570.0078438
CCTACGG150.00222353669.992163
ATACGTC150.00222353669.9921629
AGGGGGG26000.064.20434670
CAAATAC10900.062.9287261
AATACCC2300.062.3843153
CTAATAC13700.061.0515481
AATACAC25250.060.844663
TAATACA13800.060.3555532
CGTAAGA352.9535477E-760.0336541
AAATACA11650.059.778712
AATCGTC308.363615E-658.32679729
TCGAATG1450.057.93752740
CTCGAAT1350.057.0434339
CTGATTG1600.056.8686254
CGAATGC1550.056.4832741
TATTCCG252.377603E-456.04397643
TGACGTA252.3802409E-456.03140645
CCGACTT1250.056.03140647
Produced by FastQC (version 0.11.5)