FastQCFastQC Report
Wed 25 May 2016
SRR1781289_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781289_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75945
Sequences flagged as poor quality0
Sequence length76
%GC87

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3805850.11258147343472No Hit
CTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTG22702.9890052011323984No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5200.6847060372638093No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2740.3607874119428534No Hit
TCTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGT2450.32260188294160247No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2080.27388241490552373No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1760.2317466587662124No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1360.1790769635920732No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1240.16327605503983147No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGG1100.14484166172888274No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1050.13825794983211537No Hit
ATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTGGTC880.1158733293831062No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG800.10533939034827836No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAATG150.00221688570.0000170
GACTCGA150.00221688570.0000110
GTTGTGC150.00221688570.000011
TGGCGCT401.049375E-861.2513
TTGTGCG352.9388139E-759.9999962
CTTGTGC308.305244E-658.3333361
TGTGGCG308.305244E-658.33333611
CTGGCGC553.45608E-1157.27272412
CATTAAA3600.056.38889361
CGCCGTA3600.056.38889353
AGATCTC3800.056.18420840
TAGATCT3800.056.18420839
GTCTGGC252.3767882E-455.99999610
GTAGATC3900.055.64102638
TCATTAA3600.055.4166760
TATCATT3550.055.2112658
GTATCAT3500.055.057
GCCGTAT3500.055.054
TGGTCGC3950.054.9367149
ATCATTA3700.054.86486459
Produced by FastQC (version 0.11.5)