FastQCFastQC Report
Wed 25 May 2016
SRR1781289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75945
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT51726.810191586016195No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT45095.937191388504838No Hit
CAAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT40085.277503456448746No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39625.216933306998485No Hit
CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT22202.923168082164725No Hit
TCATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG19612.582131805912173No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG19122.5176114293238525No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC10001.3167423793534794No Hit
CAAATACCCATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT4290.5648824807426427No Hit
CACTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC4080.5372308907762197No Hit
CAAAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT3750.4937783922575548No Hit
CTAATACCCATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT3380.4450589242214761No Hit
AAAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT2610.3436697610112581No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC2540.3344525643557838No Hit
TCATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC2460.32391862532095594No Hit
CTAAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT2340.3081177167687142No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC1830.24096385542168677No Hit
CAAATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGT1820.23964711304233327No Hit
CTAATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGT1760.2317466587662124No Hit
ATAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT1710.225162946869445No Hit
CTAATACACATCTCCGCGCCCACGAGACGAGCACGTATCTCGTATGCCGT1700.22384620449009152No Hit
CTTATACCCATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT1660.2185792349726776No Hit
CAAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT1560.20541181117914278No Hit
CAAATACACATCTCCGCGCCCACGAGACGAGCACGTATCTCGTATGCCGT1510.1988280992823754No Hit
CTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT1500.19751135690302193No Hit
CATATACCCATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT1330.17512673645401278No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGT1220.1606425702811245No Hit
TCAAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG1210.15932582790177102No Hit
AATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT1170.1540588583843571No Hit
CTTATACACATCTCCGCGCCCACGAGACGAGCACGTATCTCGTATGCCGT1140.15010863124629664No Hit
CAAATACACAACTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT1110.14615840410823622No Hit
CATAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT1090.14352491934952927No Hit
TCATAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG1060.13957469221146881No Hit
TCATATACACATCTCCGCGCCCACGAGACGAGCACGTATCTCGTATGCCG1030.1356244650734084No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT1030.1356244650734084TruSeq Adapter, Index 2 (95% over 21bp)
CAAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGA1030.1356244650734084No Hit
CTTAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT1020.13430772269405492No Hit
CAAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT970.1277240107972875No Hit
CAAATACACATCTCCGAGCCCACGAGACGAGCACGAATCTCGTATGCCGT940.12377378365922707No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT920.12114029890052012No Hit
TCATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACG890.11719007176245967No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCAAGTATCTCGTATGCCGT870.11455658700375271No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGAATCTCGTATGCCGT850.11192310224504576No Hit
CTAATACACATCTCCGAGCCCACGAGACGAGCACGAATCTCGTATGCCGT840.11060635986569228No Hit
TCATATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCG840.11060635986569228No Hit
TCATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAG840.11060635986569228No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAACGTATCTCGTATGCCGT820.1079728751069853No Hit
CAAATACACCTCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT820.1079728751069853No Hit
CTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT810.10665613272763184No Hit
CAAATACACATCACCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT760.10007242083086443No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATTG207.8612284E-570.035654
GTATTCC207.8612284E-570.035642
CCTTGAA252.8069862E-670.035656
TCAGAAT207.8612284E-570.035653
GACGACT401.3096724E-1070.035646
ATGCATG207.8612284E-570.035654
CTGCTTC252.8069862E-670.035654
ACGACTT353.6361598E-970.035647
GAATACC207.8612284E-570.035642
CAGAATG207.8612284E-570.035654
GAAACAA150.002212414570.0355960
TTGTAAA150.002212414570.0355958
TATGATT150.002212414570.0355953
CAGATTG150.002212414570.0355954
GACGAAT150.002212414570.0355946
CTTGTAA150.002212414570.0355957
AGTCTAA150.002212414570.0355948
TGAAACA150.002212414570.0355959
TGACGAA150.002212414570.0355945
TGTAAAA150.002212414570.0355959
Produced by FastQC (version 0.11.5)