FastQCFastQC Report
Wed 25 May 2016
SRR1781288_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781288_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167754
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2392814.26374333845989No Hit
CTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTG12130.7230826090585023No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4120.245597720471643No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2210.13174052481609977No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTTC150.002221584670.012
CGCATTC200.00694174252.50000453
TGTGCAC200.00694174252.5000043
GATAGCG200.00694174252.5000047
GCCGTAT2650.048.86792454
CCGTATC2650.047.54717355
CTTAATA305.864977E-446.6666641
CGTATCA2750.045.81818456
GTATCAT2750.045.81818457
TGGTCGC3250.045.23076649
TCTCGGT3000.044.33333643
CGCCGTA3000.044.33333653
ATCATTA2850.044.21052659
GTGGTCG3250.044.15384348
AACTTAC404.5779605E-543.75000455
TAGATCT2900.043.4482839
CGGTGGT3150.043.33333646
TCGGTGG3100.042.9032345
TCGCCGT3300.041.36363252
AGATCTC3150.041.1111140
Produced by FastQC (version 0.11.5)