FastQCFastQC Report
Wed 25 May 2016
SRR1781288_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781288_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167754
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT34262.0422761901355555No Hit
CAAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT27061.6130762902821991No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT27031.6112879573661434No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22831.3609213491183518No Hit
CATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT13780.8214409194415632No Hit
TCATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG12950.7719637087640235No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG11240.6700287325488513No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC5770.3439560308547039No Hit
CACTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC2860.1704877379973056No Hit
CAAAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT2740.16333440633308297No Hit
CAAATACCCATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT2470.1472394100885821No Hit
CTAATACCCATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT2320.13829774550830382No Hit
AAAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT1960.11683775051563598No Hit
CTAAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT1910.11385719565554324No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAA150.00221475770.0543149
ATCGTAA150.002222634269.9916539
ATATCGA252.8324248E-669.9916537
TATCGTA700.059.99284438
AGATATC1000.059.4751735
CAAATAC6100.058.5001681
AATACAC13050.057.906863
AGGGGGG11000.056.6127370
CGTCATA252.3690017E-456.0769248
TATCGAA252.3865383E-455.9933238
CTAATAC6950.055.3725661
ATATCGT700.054.9934437
AAATACA6500.054.9001542
TAATACA7200.054.421732
CCGTCTA1100.054.16520747
GATATCG1050.053.31107736
TAAGCCG1250.053.2730743
CGTCTTA407.3754745E-752.57211348
ACGACTA200.006904143452.57211347
CTTAAGC200.00691230352.5564251
Produced by FastQC (version 0.11.5)