FastQCFastQC Report
Wed 25 May 2016
SRR1781287_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781287_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157174
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4098226.0742870958301No Hit
CTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTG22211.4130835888887476No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5330.3391146118314734No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2970.18896255105806303No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2350.1495158232277603No Hit
TCTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGT2180.13869978495170956No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1950.12406632140175856No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTG150.002221322470.015
TATCCTC150.002221322470.06
TAGGTGT308.346617E-658.33333239
TGTGCGC308.346617E-658.3333323
GAGTGTA200.006940930652.534
TGTAGGT407.4427044E-752.537
ATTTCGT200.006940930652.522
TCACGTA200.006940930652.524
TCGGTGG4200.052.49999645
CGTATCA3900.051.15384756
TCTCGGT3950.050.50633243
CCGTATC3900.050.2564155
GCCGTAT3900.050.2564154
AGATCTC4200.049.99999640
TAGATCT4150.049.75903739
CGCCGTA3950.049.62025553
GTATCAT4250.049.41176257
CATTAAA4150.048.91566561
ATCTCGG4200.048.33333242
ATCATTA4250.047.76470659
Produced by FastQC (version 0.11.5)