FastQCFastQC Report
Wed 25 May 2016
SRR1781287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781287_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157174
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT60783.867051802460967No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT60033.8193339865372136No Hit
CAAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT43432.7631796607581407No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39302.5004135544046724No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT26641.6949368216117169No Hit
TCATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG19101.2152137121915838No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG18991.2082150991894334No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC13290.8455596981689083No Hit
CACTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC4850.3085752096402713No Hit
CAAATACCCATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT3800.24177026734701668No Hit
CAAAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT3650.23222670416226604No Hit
CTAATACCCATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT3590.22840927888836576No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC2820.1794189878733124No Hit
CTAAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2790.17751027523636226No Hit
TCATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC2710.1724203748711619No Hit
AAAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2290.14569839795386005No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC2200.13997226004300967No Hit
CTAATACACATCTCCGCGCCCACGAGACGTTAACAGATCTCGTATGCCGT1890.1202488961278583No Hit
CTTATACCCATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT1860.11834018349090816No Hit
ATAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT1780.11325028312570783No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGA150.002222323869.9920537
ATAGGAT150.002222323869.992053
TAACGTA207.911823E-569.9920538
GTAGGAT150.002222323869.9920538
ATCGTAA207.911823E-569.9920539
TCGTATC150.002222323869.9920540
ATAACGT150.002222323869.9920537
TTCGAAG150.002222323869.9920556
AATACCC1750.065.99253
AGGGGGG20200.062.7156570
CTAATAC11850.060.2463231
CAAATAC9200.060.1018641
CACGAAT352.9616785E-759.9931839
AATACAC21200.059.0970573
TCGAATG1250.058.79331640
TAATACA12050.058.3751072
TAAGACG308.320765E-658.36384243
GAAATCG308.352241E-658.32670636
TCATATA4800.058.3267061
CGAATGC1400.057.5117641
Produced by FastQC (version 0.11.5)