FastQCFastQC Report
Wed 25 May 2016
SRR1781286_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781286_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219942
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3681116.736685126078694No Hit
CTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTG21160.9620718189340827No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5060.23006065235380238No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2860.13003428176519263No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCTA150.002222706869.99840547
ATGGTTA200.0069389852.5107465
TGTTTGA200.00694522552.4988147
AGATATC200.00694522552.4988140
GCCGTAT3750.051.33216554
CATTAAA3750.050.39885361
CGTATCA3850.049.99886756
TCATTAA3850.049.08979860
GTATCAT3900.048.46043857
CCGTATC4000.048.12390555
GTAGATC4100.047.80378738
TAGATCT4100.047.80378739
CGCCGTA4150.047.22784453
GTGTAGA4550.046.92201236
TCTCGGT4250.046.116643
TGTAGAT4650.045.91293737
TATCATT4050.045.80142658
ATCATTA4150.045.54113459
AGATCTC4300.044.76642640
TGTGTAG4650.043.65492235
Produced by FastQC (version 0.11.5)