FastQCFastQC Report
Wed 25 May 2016
SRR1781286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781286_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219942
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT55652.5302125105709687No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT48782.217857435141992No Hit
CAAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT39931.8154786261832665No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35651.620881868856335No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT22291.0134490001909593No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG18040.8202162388265998No Hit
TCATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG17220.7829336825162998No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC11460.5210464577024853No Hit
CACTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC4340.19732474925207555No Hit
CAAATACCCATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT4270.19414209200607432No Hit
CTAATACCCATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT3920.17822880577606826No Hit
CAAAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT3250.147766229278628No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC2300.1045730237971829No Hit
CTAAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT2290.10411835847632558No Hit
AAAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT2260.10275436251375362No Hit
TCATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC2230.10139036655118167No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG150.00221692470.0443843
CAAATAC9750.063.894321
AAATACA9850.062.1655732
AATACAC20300.060.328173
AATCGTA352.9658258E-759.9970838
AATACCC2050.059.7395973
AGGGGGG19050.059.3274570
CTAATAC10600.058.4405441
TATGACG1650.057.3090443
CTCGAAT1100.057.26993639
TAATACA10750.056.635522
TAAGCCG509.713403E-1056.035543
TCGTATC252.3873466E-455.9972740
CATTATA509.786163E-1055.9845352
TCGAAAA252.3900316E-455.98453534
TCGAATG1200.055.41396740
GTCTTCA1100.054.1375249
TCTCGAA1250.053.19740738
CGTCTTA407.4005766E-752.55720548
AGCTTGA1400.052.5213355