FastQCFastQC Report
Wed 25 May 2016
SRR1781285_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781285_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178356
Sequences flagged as poor quality0
Sequence length76
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5245129.40803785687053No Hit
CTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTG28071.573818654825181No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5570.31229675480499675No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4470.25062235080400996No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2780.1558680392024939No Hit
TCTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGT2630.14745789320235933No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1990.1115746036017852No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG301.0193253E-770.02
GTCGTAC150.002221815570.016
GTTGCTC150.002221815570.03
ATTAAAA4850.056.2886662
CGTATCA4800.056.14583256
GCCGTAT4800.056.14583254
CATTAAA4900.055.71428761
CCGTATC4850.055.5670155
TATCATT4950.055.15151658
TCATTAA5000.054.660
ATCATTA4950.054.44444759
GTATCAT5200.053.1730857
TTAAAAA5250.052.66666863
AATCCCC200.006942460352.59
AAGAGAC200.006942460352.55
TAAGTGG200.006942460352.538
TGAAATC200.006942460352.525
TACATCG200.006942460352.552
CAATGCA200.006942460352.523
CCAGTGA200.006942460352.527
Produced by FastQC (version 0.11.5)